Elucidating the molecular mechanism of the migration and invasion is critical for identifying novel therapeutic targets and may significantly improve the prognosis of colorectal cancer. Emerging evidence suggests an involvement of dysregulated microRNAs in the process of tumorigenesis. Here, we show that miR-1225-5p prevents migration and invasion of colorectal cancer cells. Overexpression of miR-1225-5p significantly decreases the expression of Matrix Metalloproteases (MMPs)-1, 3, and 9. Knockdown of miR-1225-5p elevates ROS level via regulating Keap1/Nrf2 pathway. Furthermore, miR-1225-5p attenuates IL-17A-induced p38/AP-1-signaling pathway by suppressing IL-17RA expression. We also examined the biological effects of Rosmarinic acid (RA) on metastatic colorectal cancer cells. RA inhibited EMT via the p38/AP-1 signaling, and miR-1225-5p is essential for RA-mediated anti-metastatic effects.

中文翻译：

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迷迭香酸通过 miR-1225-5p 在结直肠癌细胞中抑制迁移、侵袭和 p38/AP-1 信号传导

阐明迁移和侵袭的分子机制对于确定新的治疗靶点至关重要，并可能显着改善结直肠癌的预后。新出现的证据表明，失调的 microRNA 参与了肿瘤发生的过程。在这里，我们表明 miR-1225-5p 可防止结直肠癌细胞的迁移和侵袭。miR-1225-5p 的过表达显着降低基质金属蛋白酶 (MMPs)-1、3 和 9 的表达。敲除 miR-1225-5p 通过调节 Keap1/Nrf2 通路提高 ROS 水平。此外，miR-1225-5p 通过抑制 IL-17RA 表达减弱 IL-17A 诱导的 p38/AP-1 信号通路。我们还研究了迷迭香酸 (RA) 对转移性结直肠癌细胞的生物学作用。RA 通过 p38/AP-1 信号抑制 EMT，