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Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping.
Functional Plant Biology ( IF 2.6 ) Pub Date : 2020-08-24 , DOI: 10.1071/fp20167
Muhammad A Asif 1 , Melissa Garcia 2 , Joanne Tilbrook 1 , Chris Brien 3 , Kate Dowling 4 , Bettina Berger 5 , Rhiannon K Schilling 1 , Laura Short 1 , Christine Trittermann 1 , Matthew Gilliham 6 , Delphine Fleury 7 , Stuart J Roy 8 , Allison S Pearson 9
Affiliation  

Bread wheat (Triticum aestivum L.) is one of the most important food crops, however it is only moderately tolerant to salinity stress. To improve wheat yield under saline conditions, breeding for improved salinity tolerance of wheat is needed. We have identified nine quantitative trail loci (QTL) for different salt tolerance sub-traits in a recombinant inbred line (RIL) population, derived from the bi-parental cross of Excalibur × Kukri. This population was screened for salinity tolerance subtraits using a combination of both destructive and non-destructive phenotyping. Genotyping by sequencing (GBS) was used to construct a high-density genetic linkage map, consisting of 3236 markers, and utilised for mapping QTL. Of the nine mapped QTL, six were detected under salt stress, including QTL for maintenance of shoot growth under salinity (QG(1-5).asl-5A, QG(1-5).asl-7B) sodium accumulation (QNa.asl-2A), chloride accumulation (QCl.asl-2A, QCl.asl-3A) and potassium : sodium ratio (QK:Na.asl-2DS2). Potential candidate genes within these QTL intervals were shortlisted using bioinformatics tools. These findings are expected to facilitate the breeding of new salt tolerant wheat cultivars.



中文翻译:

使用破坏性和非破坏性表型鉴定小麦 RIL 作图群体中的耐盐性 QTL。

面包小麦 ( Triticum aestivumL.) 是最重要的粮食作物之一,但它对盐胁迫的耐受性中等。为了提高盐碱条件下的小麦产量,需要进行提高小麦耐盐性的育种。我们已经确定了重组自交系 (RIL) 群体中不同耐盐亚性状的九个定量追踪基因座 (QTL),该群体源自 Excalibur × Kukri 的双亲杂交。使用破坏性和非破坏性表型的组合筛选该群体的耐盐性亚性。测序基因分型(GBS)用于构建由3236个标记组成的高密度遗传连锁图谱,并用于定位QTL。在绘制的 9 个 QTL 中,有 6 个在盐胁迫下检测到,其中包括盐胁迫下维持枝条生长的 QTL ( QG (1-5 ) .asl - 5A , QG ( 1-5 ) .asl - 7B ) 钠积累 ( QNa.asl - 2A ) 氯化物积累 ( QCl.asl - 2A , QCl.asl - 3A ) 和钾钠比( QK Na.asl - 2DS2)。使用生物信息学工具将这些 QTL 区间内的潜在候选基因列入候选名单。这些发现有望促进新的耐盐小麦品种的培育。

更新日期:2020-08-25
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