Nucleation and growth inhibition of biological minerals by cementum attachment protein-derived peptide (CAP-pi).,Journal of Peptide Science

当前位置： X-MOL 学术 › J. Pept. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Nucleation and growth inhibition of biological minerals by cementum attachment protein-derived peptide (CAP-pi).
Journal of Peptide Science ( IF 1.8 ) Pub Date : 2020-08-24 , DOI: 10.1002/psc.3282
Gonzalo Montoya ₁ , Kevin Lopez ₁ , Jesús Arenas ₂ , Claudia Zamora ₁ , Lía Hoz ₁ , Enrique Romo ₁ , Karina Jiménez ₃ , Higinio Arzate ₁

Affiliation

Biomineralization is a highly regulated process where proteins/peptides‐crystal interactions contribute to the shaping, phasing and aggregation of minerals. We have identified and synthesized a cementum attachment protein‐derived peptide (CAP‐pi), which corresponds to amino acids 40–53 of the N‐terminal CAP domain (MASSDEDGTNGGAS) and its phosphorylated variant (MASpSpDEDGTNGGASp) (CAP‐pip). The peptide is composed of polar and negatively charged amino acids, which are disordered, according to in silico analysis. Our results show that CAP‐pi inhibits hydroxyapatite (HA) formation and growth. However, it possesses low capacity to inhibit calcium oxalate crystal growth. CAP‐pip showed a stronger inhibitory effect on the formation and growth of HA. As well as a high capacity to inhibit calcium oxalate monohydrate growth, mainly due to adsorption on specific growth faces. Small peptides have many advantages over the full‐size protein, including low‐cost production and modulation characteristics that allow for structural changes. Our findings suggest that CAP‐pip‐derived peptide could possess therapeutic potential to prevent or treat pathological calcifications such as renal stones and vascular calcification.

中文翻译：

牙骨质附着蛋白衍生肽（CAP-pi）对生物矿物质的成核和生长抑制作用。

生物矿化是一个高度受控的过程，其中蛋白质/肽-晶体相互作用有助于矿物质的形成，定相和聚集。我们已经鉴定并合成了牙骨质附着蛋白衍生肽（CAP-pi），它对应于N末端CAP结构域（MASSDEDGTNGGAS）40-53位氨基酸及其磷酸化变体（MASpSpDEDGTNGGASp）（CAP-pip）。该肽是根据由极性和带负电荷的氨基酸，这是无序的，在计算机芯片上分析。我们的结果表明，CAP-pi抑制羟基磷灰石（HA）的形成和生长。但是，其抑制草酸钙晶体生长的能力低。CAP-pip对HA的形成和生长表现出更强的抑制作用。以及高抑制草酸钙一水合物生长的能力，这主要是由于特定生长面上的吸附。小肽相对于全尺寸蛋白质具有许多优势，包括低成本的生产和可改变结构的调节特性。我们的发现表明，CAP肽可能具有预防或治疗病理性钙化（例如肾结石和血管钙化）的治疗潜力。

更新日期：2020-08-24

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11