This study evaluated the biological behavior of the coffee compounds Trigonelline (T), chlorogenic acid (C), and nicotinic acid (N), correlating with their release from a resin matrix. Minimum inhibitory concentration (MIC) was evaluated against Streptococcus mutans UA159, and cytotoxicity was assessed by methyl tetrazolium salt on OD‐21 cells. Resin matrices (bisphenol A‐glycidyl‐dimethacrylate/triethylene glycol‐dimethacrylate 70/30 wt%, camphorquinone/ethyl 4‐dimethyl aminobenzoate 0.5/1 wt%) were doped with coffee compounds in different concentrations (10/20/30/40/50 wt%), performing 15 groups (T10‐T50, C10‐C50, N10‐N50), and a control group with no coffee compound. Degree of conversion (DC%) was analyzed by Fourier transform infrared spectroscopy. Antimicrobial properties were evaluated by bioluminescence (Luciferase assay). The release from loaded matrices was analyzed over time (24 hr, 6, 14, 21 and 28 days), using high‐performance liquid chromatography (HPLC). Data were submitted to ANOVA/Tukey's test (α = 0.05). MIC for T and C was 6 mg/ml, and 4 mg/ml for N. None of them were cytotoxic. Only T50 and C50 showed lower DC% than control (α < 0.05). Some groups (T30/T40/T50/C40/C50/N50) were strongly antimicrobial, reducing bacterial activity approximately five times compared to control (α < 0.05). For T30, T40, T50, C40, and C50, the HPLC showed a release above or closer to MIC values mainly in 24 hr, but for N50, up to 28 days. In conclusion, the coffee compounds presented antimicrobial activity, depending on their concentration when added in resin matrices, being found a correlation with their release.

中文翻译：

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负载咖啡化合物的树脂基质的抗菌潜力。


这项研究评估了咖啡化合物葫芦巴碱 (T)、绿原酸 (C) 和烟酸 (N) 的生物行为，与其从树脂基质中的释放相关。评估了针对变形链球菌UA159 的最低抑菌浓度 (MIC)，并通过甲基四唑盐评估了 OD-21 细胞的细胞毒性。树脂基质（双酚 A-二甲基丙烯酸缩水甘油酯/三甘醇-二甲基丙烯酸酯 70/30 wt%，樟脑醌/4-二甲基氨基苯甲酸乙酯 0.5/1 wt%）掺杂有不同浓度（10/20/30/40/ 50 wt%），进行 15 组（T10-T50、C10-C50、N10-N50），以及不含咖啡化合物的对照组。通过傅里叶变换红外光谱分析转化率(DC%)。通过生物发光（荧光素酶测定）评估抗菌特性。使用高效液相色谱 (HPLC) 对加载基质的释放随时间（24 小时、6、14、21 和 28 天）进行分析。数据提交至 ANOVA/Tukey 检验 (α = 0.05)。 T 和 C 的 MIC 为 6 mg/ml，N 的 MIC 为 4 mg/ml。它们都没有细胞毒性。仅 T50 和 C50 显示出低于对照的 DC% (α < 0.05)。一些组 (T30/T40/T50/C40/C50/N50) 具有很强的抗菌作用，与对照组 (α < 0.05) 相比，细菌活性降低了大约五倍。对于 T30、T40、T50、C40 和 C50，HPLC 显示主要在 24 小时内释放高于或接近 MIC 值，但对于 N50，长达 28 天。总之，咖啡化合物表现出抗菌活性，具体取决于它们添加到树脂基质中时的浓度，并被发现与其释放相关。