The development of novel materials with effective defect‐repairing properties will help avoid subtotal gastrectomy in patients with large gastric perforations. We prepared perfused decellularized gastric matrix (PDGM) and analyzed its components, spatial structure, biomechanics, cytotoxicity, and histocompatibility to validate its efficacy in the repair of gastric perforation. PDGM retained large amounts of gastric extracellular matrix, while residual glandular cells and muscle fibers were not found. The spatial structure of the tissue was well preserved, while the DNA and glycosaminoglycan contents were significantly decreased compared with normal gastric tissue (p < .01). There was no obvious deformation of the spatial structure and tissue elasticity of PDGM after sterilization by Cobalt‐60 irradiation. The PDGM had good histocompatibility. PDGM was then used to repair a rat gastric perforation model. Radiography of the upper gastrointestinal tract at 24 hr postoperatively revealed no contrast agent leakage. There was evidence of early fibroblast proliferation, which was complicated by capillary regeneration. The hyperplastic gastric gland was slightly disarranged after repair. Defects of the muscular layer also healed a little with the regeneration process. PDGM is a nontoxic biocompatible biological mesh that may be useful for repairing relatively large gastric defects.

中文翻译：

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脱细胞胃基质作为胃穿孔修复的网状结构。

开发具有有效缺陷修复特性的新型材料将有助于避免大胃穿孔患者的胃大部切除术。我们制备了灌注脱细胞胃基质 (PDGM) 并分析了其成分、空间结构、生物力学、细胞毒性和组织相容性，以验证其在胃穿孔修复中的功效。PDGM 保留了大量的胃细胞外基质，而没有发现残留的腺细胞和肌纤维。组织空间结构保存完好，与正常胃组织相比，DNA和糖胺聚糖含量显着降低（p < .01)。Cobalt-60辐照灭菌后PDGM的空间结构和组织弹性没有明显变形。PDGM具有良好的组织相容性。然后使用 PDGM 修复大鼠胃穿孔模型。术后 24 小时的上消化道 X 光片显示没有造影剂泄漏。有早期成纤维细胞增殖的证据，其因毛细血管再生而复杂化。修复后增生的胃腺略有紊乱。肌肉层的缺陷也随着再生过程而愈合了一点。PDGM 是一种无毒的生物相容性生物网，可用于修复相对较大的胃缺陷。