Copper oxide nanoparticles (CuONPs) were phytosynthesized by Laurus nobilis leaf extract, which was used as a reducing and capping agent. UV-vis spectroscopy was applied, and the spectrum of CuONPs gave a peak around 300 and 325 nm. An intense Fourier transform infrared spectroscopy between 4000 and 500 cm⁻¹ wavelengths exhibited exterior functional groups of CuONPs. The results of scanning electron microscopy and transmission electron microscopy revealed that the green synthesized CuONPs were spherical in shape with sizes between 90 and 250 nm. Antibacterial activity of CuONPs was evaluated against both Gram-positive and Gram-negative bacteria. Brilliant Blue R-250 was employed in the dye decolorization studies, and CuONPs achieved 69% decolorization in 60 Min. The antioxidant activity of CuONPs was calculated by analyzing total phenolic compounds and flavonoid content. Furthermore, the reducing power of extract and nanoparticles was determined. Total phenolic compounds of CuONPs were determined as 6.7 µg of pyrocatechol equivalent/mg, while total flavonoids were measured as 236.62 µg catechin/mg sample. Results indicated that the method of CuONP formation is simple and low cost and the phytosynthesized CuONPs had antibacterial, antioxidant, and dye decolorization activity.

中文翻译：

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CuONPs 通过 Laurus nobilis 的植物合成：抗氧化含量、抗菌活性和染料脱色潜力的测定

氧化铜纳米粒子 (CuONPs) 由月桂叶提取物合成，用作还原剂和封端剂。应用紫外-可见光谱，CuONPs 的光谱在 300 和 325 nm 附近出现峰值。4000 到 500 cm ^-1之间的强傅立叶变换红外光谱波长表现出CuONPs的外部官能团。扫描电镜和透射电镜结果表明，绿色合成的 CuONPs 呈球形，尺寸在 90 到 250 nm 之间。CuONPs 对革兰氏阳性菌和革兰氏阴性菌的抗菌活性进行了评估。亮蓝 R-250 用于染料脱色研究，CuONPs 在 60 分钟内实现了 69% 的脱色。通过分析总酚类化合物和类黄酮含量来计算 CuONPs 的抗氧化活性。此外，还测定了提取物和纳米颗粒的还原能力。CuONPs 的总酚类化合物测定为 6.7 µg 焦儿茶酚当量/mg，而总黄酮类化合物测定为 236.62 µg 儿茶素/mg 样品。