There is considerable evidence that abiotic factors have an important influence on the microbial diversity of natural ecosystems. In the present study, we tested the hypothesis that rhizospheric and endophytic micro-organisms isolated from the roots of Hymenaea courbaril L. in two biomes with distinct ecological characteristics – the Pantanal wetland and the Cerrado savanna – present distinct functional traits. We also evaluated the functional diversity (H′) of the microbiota and the potential effects of soil parameters, such as Organic Material (OM), cation exchange capacity (CEC), and nutrient concentrations, on the relative abundance of the micro-organisms with functional traits such as the solubilization of phosphates (CaHPO₄, AlPO₄, and FePO₄), the synthesis of indole-3-acetic acid (IAA) and the ACC deaminase enzyme, and antibiosis to the phytopathogens Fusarium sp., Sclerotinia sclerotiorum, and Rhizoctonia sp. A higher percentage of the micro-organisms from the Pantanal (in comparison with the Cerrado) presented intense or very intense function in the synthesis of IAA and antibiosis, with a higher degree of specialization (reduced functional diversity) in the former trait (H’ = 0.73) and a lower degree in the latter (H’ = 0.91 for antibiosis to Fusarium sp.; H’ = 0.97 for S. sclerotiorum, and H’ = 0.80 for Rhizoctonia sp.). The micro-organisms of the Cerrado were more effective in the solubilization of phosphates, and the mean concentrations of P solubilized by these micro-organisms were higher than those observed in the isolates from the Pantanal, when the sources were AlPO₄ and FePO₄, which indicates a potential relationship between the high concentrations of Al and Fe found naturally in the soils of the Cerrado and the functional traits of its endemic microbiota. We also observed high levels of diversity in the functional traits of the Cerrado micro-organisms involved in solubilization (H’ = 1.09 for CaHPO₄, H’ = 1.05 for AlPO₄, and H’ = 1.09 for FePO₄). All the isolates from both biomes synthetized ACC deaminase very intensively. In the Pantanal, soil parameters such as pH, OM, CEC, and the levels of Mn and Fe, as well as the Fe concentrations in the root and aerial portion of the plant affected the relative abundance of the micro-organisms that had efficient functional traits. In the Cerrado soil, the parameters pH, CEC, and the Mn, Mg, and Fe concentrations, as well as the level of Fe in the aerial part of the plant all affected the relative abundance. We proved our hypothesis on the specialization of the micro-organisms for specific functional traits, and also that the functionality of the symbiotic microbiota is influenced by environmental parameters. The present study provides important insights into the interactions between the soil and the endophytic root and rhizospheric micro-organisms isolated from H. courbaril L. in the Pantanal and Cerrado biomes. These findings will support future studies that will better define the environmental factors that influence the expression of different functional traits by the micro-organisms in the microbiome.

有大量证据表明，非生物因素对自然生态系统的微生物多样性具有重要影响。在本研究中，我们测试了以下假说：在两个具有明显生态特征的生物群落中，从鬣狗科库巴的根中分离出的根际和内生微生物–潘塔纳尔湿地和塞拉多热带稀树草原–表现出独特的功能性状。我们还评估了微生物群的功能多样性（H'）以及土壤参数（如有机材料（OM），阳离子交换容量（CEC）和养分浓度）对微生物相对丰度的潜在影响。功能性状，例如磷酸盐（CaHPO ₄，AlPO ₄，FePO ₄），吲哚-3-乙酸（IAA）和ACC脱氨酶的合成以及对植物病原镰刀菌（Fusarium sp。）的抗菌作用。，核盘菌和核糖菌。来自潘塔纳尔氏菌的微生物的比例较高（与Cerrado相比），在IAA的合成和抗微生物作用中表现出强烈或非常强烈的功能，而前者的性状（H'  = 0.73），而后者的程度较低（ 对于镰孢镰刀菌的抗生性，H' = 0.91 ；硬核链球菌H'  = 0.97 ，而H' 对于Rhizoctonia sp。= 0.80 ）。当来源为AlPO ₄和FePO _4时，塞拉多微生物对磷酸盐的溶解更有效，并且这些微生物溶解的P的平均浓度高于潘塔纳尔分离物中的P。这表明塞拉多土壤中天然存在的高浓度铝和铁与其特有微生物群落的功能性状之间存在潜在的关系。我们还观察到参与溶解（塞拉多微生物的功能性状高多样性水平H“  = 1.09使用CaHPO ₄，H”  = 1.05的AlPO ₄，和 FePO _4的H' = 1.09）。来自两个生物群落的所有分离物都非常密集地合成了ACC脱氨酶。在潘塔纳尔湿地，土壤参数（例如pH，OM，CEC，锰和铁的含量以及植物根部和空中部分的铁浓度）影响了具有有效功能的微生物的相对丰度。特质。在Cerrado土壤中，pH，CEC，Mn，Mg和Fe的浓度参数以及植物地上部分的Fe含量都会影响相对丰度。我们证明了关于特定功能性特征的微生物专业化的假设，而且共生微生物的功能性受环境参数的影响。潘塔纳尔湿地和塞拉多生物群落中的H. courbaril L.。这些发现将支持将来的研究，这些研究将更好地定义环境因素，这些环境因素会影响微生物组中微生物的不同功能性状的表达。