Quantifying and characterizing Extracellular Polymeric Substances (EPS) and especially exopolysaccharides (EPSac) is an issue for understanding the hydro-physical and biological functioning of the rhizosphere. However, few comparative studies of extraction techniques have been carried out on soils and none on calcareous Mediterranean soils. Three soil-bound EPS extraction techniques, i.e. Cation Exchange Resin (CER), EDTA and NaOH + Formaldhyde (NaOH + F) were compared on three contrasted Mediterranean soils. CER presented the lowest extraction efficiency of EPSac, but also the lowest contamination of EPS by extractants and extracellular compounds. Contamination with intracellular compounds was low and similar with the three methods. Mid-Infrared (MIR) spectra enabled the best discrimination of the EPS extracts when they were prepared with CER. CER is then identified to be the suitable extraction technique of EPS (including EPSac) from soils, including calcareous soils. This technique was applied on rhizospheric and bulk soils harvested in an experimental field of tomato cultivation. A rhizospheric effect was highlighted during the growth of plants of two cultivars with both the soil-bound EPSac amounts (total sugar equivalent of extracted EPS) and the MIR spectra of extracted EPS. Extraction of soil-bound EPS and their further analysis by spectral and chemometric approaches is a promising way for relating EPS chemical characteristics to their biological and hydric impacts within the plant rhizosphere in a context of agro-ecological transition and climatic change.

量化和表征细胞外聚合物（EPS），尤其是胞外多糖（EPSac）是了解根际的水物理和生物学功能的一个问题。但是，很少在土壤上进行提取技术的比较研究，而在钙质地中海土壤上没有进行比较研究。在三种对比的地中海土壤上比较了三种结合土壤的EPS提取技术，即阳离子交换树脂（CER），EDTA和NaOH +甲醛（NaOH + F）。CER表现出EPSac的最低提取效率，但提取剂和细胞外化合物对EPS的污染也最低。细胞内化合物的污染很低，并且与三种方法相似。用CER制备EPS提取物时，中红外（MIR）光谱可以最好地区分它们。然后，将CER确定为从包括钙质土壤在内的土壤中提取EPS（包括EPSac）的合适技术。这项技术被应用于番茄栽培实验领域中的根际土壤和块状土壤。在两个品种的植物生长过程中，根际EPSac量（提取的EPS的总糖当量）和提取的EPS的MIR光谱都突出了根际效应。在农业生态转变和气候变化的背景下，提取结合土壤的EPS并通过光谱和化学计量方法进行进一步分析是将EPS化学特性与其在植物根际内的生物和水力影响联系起来的一种有前途的方式。这项技术被应用于在番茄栽培实验领域中收获的根际土壤和块状土壤。在两个品种的植物生长过程中，根际的EPSac量（提取的EPS的总糖当量）和提取的EPS的MIR光谱都突出了根际效应。在农业生态过渡和气候变化的背景下，提取结合土壤的EPS并通过光谱和化学计量方法进行进一步分析是将EPS化学特性与其在植物根际内的生物和水力影响联系起来的一种有前途的方式。这项技术被应用于番茄栽培实验领域中的根际土壤和块状土壤。在两个品种的植物生长过程中，根际的EPSac量（提取的EPS的总糖当量）和提取的EPS的MIR光谱都突出了根际效应。在农业生态转变和气候变化的背景下，提取结合土壤的EPS并通过光谱和化学计量方法进行进一步分析是将EPS化学特性与其在植物根际内的生物和水力影响联系起来的一种有前途的方式。在两个品种的植物生长过程中，根际的EPSac量（提取的EPS的总糖当量）和提取的EPS的MIR光谱都突出了根际效应。在农业生态转变和气候变化的背景下，提取结合土壤的EPS并通过光谱和化学计量方法进行进一步分析是将EPS化学特性与其在植物根际内的生物和水力影响联系起来的一种有前途的方式。在两个品种的植物生长过程中，根际EPSac量（提取的EPS的总糖当量）和提取的EPS的MIR光谱都突出了根际效应。在农业生态转变和气候变化的背景下，提取结合土壤的EPS并通过光谱和化学计量方法进行进一步分析是将EPS化学特性与其在植物根际内的生物和水力影响联系起来的一种有前途的方式。