当前位置: X-MOL 学术Physiol. Mol. Plant Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Variation in several pathogenesis - related (PR) protein genes in wheat (Triticum aestivum) involved in defense against Puccinia striiformis f. sp. tritici
Physiological and Molecular Plant Pathology ( IF 2.8 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.pmpp.2020.101545
Samar M. Esmail , Aziza A. Aboulila , Diaa Abd El-Moneim

Abstract Proteins participate in defense mechanisms after a pathogen attacks a host. Pathogenesis-related (PR) proteins in wheat plants play the important role of defense against Puccinia striiformis f. sp. Tritici. This study uses various analytic methods to describe the expression of five (PR) protein genes (PR1, PR2, PR4, PR9 and PR10) and genetic polymorphism among sixteen wheat genotypes to understand the mechanisms underlying of PR genes in Egyptian wheat compared with the Yr genotype. The PR protein genes were semi-quantitatively and quantitatively assessed in four selected wheat genotypes; two resistant (Misr-3 and Yr-29) and two susceptible (Sids-12 and Avocet-S). Reverse transcriptase PCR analysis results indicated that all PR protein genes exhibited different expression levels among the four studied genotypes and overexpression at the resistant genotypes in the different time points of Pst inoculation. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the expression of PR protein genes was elevated in adult plant resistant (APR) genotypes. Plant stage in combination with the infection process by stripe rust provided even greater upregulation in the expression of PR genes and enhanced induction of defense enzyme activities in the same sample at different time points for various wheat genotypes.

中文翻译:

小麦(Triticum aestivum)中几种发病机制相关(PR)蛋白基因的变异,这些基因参与防御条状锈病菌(Puccinia striiformis f)。sp. 小麦

摘要 蛋白质在病原体攻击宿主后参与防御机制。小麦植株中的病原相关 (PR) 蛋白在防御条状锈病菌中发挥重要作用。sp. 小麦。本研究使用多种分析方法描述了 5 个(PR)蛋白基因(PR1、PR2、PR4、PR9 和 PR10)的表达和 16 个小麦基因型之间的遗传多态性,以了解与 Yr 相比埃及小麦中 PR 基因的潜在机制。基因型。PR蛋白基因在四种选定的小麦基因型中进行了半定量和定量评估;两种抗性(Misr-3 和 Yr-29)和两种易感性(Sids-12 和 Avocet-S)。逆转录酶PCR分析结果表明,在Pst接种的不同时间点,所有PR蛋白基因在四种研究基因型中均表现出不同的表达水平,并在抗性基因型中过表达。定量实时聚合酶链反应 (qRT-PCR) 分析表明,成年植物抗性 (APR) 基因型中 PR 蛋白基因的表达升高。植物阶段结合条锈病的侵染过程提供了更大的 PR 基因表达上调,并增强了同一样品中不同小麦基因型在不同时间点的防御酶活性诱导。定量实时聚合酶链反应 (qRT-PCR) 分析表明,成年植物抗性 (APR) 基因型中 PR 蛋白基因的表达升高。植物阶段结合条锈病的侵染过程提供了更大的 PR 基因表达上调,并增强了同一样品中不同小麦基因型在不同时间点的防御酶活性诱导。定量实时聚合酶链反应 (qRT-PCR) 分析表明,成年植物抗性 (APR) 基因型中 PR 蛋白基因的表达升高。植物阶段结合条锈病的侵染过程提供了更大的 PR 基因表达上调,并增强了同一样品中不同小麦基因型在不同时间点的防御酶活性诱导。
更新日期:2020-12-01
down
wechat
bug