Chitosan oligosaccharides (COS) can elicit plant immunity and defence responses in rice plants, but exactly how this promotes plant growth remains largely unknown. Herein, we explored the effects of 0.5 mg/L COS on plant growth promotion in rice seedlings by measuring root and stem length, investigating biochemical factors in whole plants via proteomic analysis, and confirming upregulated and downregulated genes by real-time quantitative PCR. Pathway enrichment results showed that COS promoted root and stem growth, and stimulated metabolic (biosynthetic and catabolic processes) and photosynthesis in rice plants during the seedling stage. Expression levels of genes related to chlorophyll a-b binding, RNA binding, catabolic processes and calcium ion binding were upregulated following COS treatment. Furthermore, comparative analysis indicated that numerous proteins involved in the biosynthesis, metabolic (catabolic) processes and photosynthesis pathways were upregulated. The findings indicate that COS may upregulate calcium ion binding, photosynthesis, RNA binding, and catabolism proteins associated with plant growth during the rice seedling stage.

中文翻译：

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壳寡糖处理后水稻幼苗生化因子的数据独立获取蛋白质组学分析

壳聚糖寡糖 (COS) 可以在水稻中引发植物免疫和防御反应，但究竟如何促进植物生长仍然未知。在此，我们通过测量根和茎的长度，通过蛋白质组学分析调查整株植物的生化因素，并通过实时定量 PCR 确认上调和下调基因，探讨了 0.5 mg/L COS 对水稻幼苗植物生长促进的影响。通路富集结果表明，COS 促进根和茎的生长，并刺激水稻苗期的代谢（生物合成和分解代谢过程）和光合作用。COS 处理后，与叶绿素 ab 结合、RNA 结合、分解代谢过程和钙离子结合相关的基因表达水平上调。此外，比较分析表明，参与生物合成、代谢（分解代谢）过程和光合作用途径的许多蛋白质被上调。研究结果表明，COS 可能上调与水稻幼苗期植物生长相关的钙离子结合、光合作用、RNA 结合和分解代谢蛋白。