The scientific interest on the plant Cannabis sativa L., and in particular on its non-psychoactive or fibre-type variety (hemp), has been highly increasing in recent years, due to the pharmaceutical and nutraceutical potential of its bioactive compounds. This plant is indeed characterized by a very rich chemical composition, which encompasses different classes of constituents, such as cannabinoids and terpenes. In this context, the bioanalytical testing of hemp extracts can be difficult and time-consuming. Effect-directed analysis (EDA) by the combination of high-performance thin-layer chromatography (HPTLC) with biological and enzymatic assays represents one of the latest tools available for the rapid bioprofiling of complex matrices, such as plant extracts. In this ambit, the aim of this project was the non-targeted screening of inflorescence extracts from ten different hemp varieties for components exhibiting radical scavenging, antibacterial, enzyme inhibiting and estrogen-like effects. By HPTLC-EDA, the hemp samples exhibited strong antibacterial activities against both Gram-positive Bacillus subtilis and especially Gram-negative Aliivibrio fischeri bacteria, and also estrogen-like activity. They also inhibited α- and β-glucosidase, tyrosinase and acetylcholinesterase. The characterization of two prominently multipotent bioactive compound zones was finally achieved by HPTLC-HRMS and preliminary assigned as cannabidiolic acid and cannabidivarinic acid.

对植物大麻的科学兴趣近年来，由于其生物活性化合物的药学和营养学潜力，L。尤其是其非精神活性或纤维类型品种（大麻）已经高度增加。该植物的确以非常丰富的化学成分为特征，其中包含不同种类的成分，如大麻素和萜烯。在这种情况下，大麻提取物的生物分析测试可能既困难又费时。通过将高性能薄层色谱（HPTLC）与生物学和酶促分析相结合的效果导向分析（EDA）代表了可用于对复杂基质（例如植物提取物）进行快速生物概况分析的最新工具之一。在这个范围内 该项目的目的是针对十种不同大麻品种的花序提取物进行针对性筛选，这些成分具有自由基清除，抗菌，酶抑制和类似雌激素的作用。通过HPTLC-EDA，大麻样品对两种革兰氏阳性菌均显示出强大的抗菌活性。枯草芽孢杆菌，特别是革兰氏阴性费氏杆菌细菌，以及类似雌激素的活性。它们还抑制α-和β-葡萄糖苷酶，酪氨酸酶和乙酰胆碱酯酶。最终，通过HPTLC-HRMS实现了两个显着的多能生物活性化合物区域的表征，并初步指定为大麻二酚酸和大麻二炔酸。