The light spectrum quality is an important signal for plant growth and development. We evaluated the effects of different light spectra on the in vitro shoot development of Cedrela fissilis and its proteomic and polyamine (PA) profiles. Cotyledonary and apical nodal segments were grown under different light emitting diodes (LED) and fluorescent lamps. Shoots from cotyledonary nodal segments cultured with 6-benzyladenine (BA) that were grown under W_mB_dR LED showed increased length and higher fresh and dry matter compared to shoots grown under fluorescent lamps. A nonredundant protein databank generated by transcriptome sequencing and the de novo assembly of C. fissilis improved, and almost doubled, the protein identification compared to a Citrus sinensis databank. A total of 616 proteins were identified, with 23 up- and 103 down-accumulated in the shoots under W_mB_dR LEDs compared to fluorescent lamps. Most differentially accumulated proteins in shoots grown under the WmBdR LED lamp treatment compared to the fluorescent lamp treatment are involved in responding to metabolic processes, stress, biosynthetic and cellular protein modifications, and light stimulus processes. Among the proteins, the up-accumulation of argininosuccinate synthase was associated with an increase in the free putrescine content and, consequently, with higher shoot elongation under W_mB_dR LED. The down-accumulation of calreticulin, heat shock proteins, plastid-lipid-associated protein, ubiquitin-conjugating enzymes, and ultraviolet-B receptor UVR8 isoform X1 could be related to the longer shoot length noted under LED treatment. This study provides important data related to the effects of the light spectrum quality on in vitro morphogenesis through the modulation of specific proteins and free putrescine biosynthesis in C. fissilis, an endangered wood species from the Brazilian Atlantic Forest of economic and ecological relevance. The nonredundant protein databank of C. fissilis is available via ProteomeXchange under identifier PXD018020.

光谱质量是植物生长发育的重要信号。我们评估了不同光谱对Cedrela fissilis的体外芽发育及其蛋白质组学和多胺（PA）谱的影响。子叶节和根节节在不同的发光二极管（LED）和荧光灯下生长。与在荧光灯下生长的芽相比，用6-甲基腺嘌呤（BA）培养的子叶节段的芽在W _m B _d R LED下生长，其长度增加，新鲜和干物质增加。通过转录组测序和菲氏梭菌的从头组装产生的非冗余蛋白质数据库与柑桔数据库相比，蛋白质的识别能力提高了，几乎翻了一番。与荧光灯相比，W _m B _d R LED下的芽中总共鉴定出616种蛋白质，其中芽中的23种上聚和103种下聚。与荧光灯处理相比，在WmBdR LED灯处理下生长的芽中差异最大的蛋白质参与了对代谢过程，应激，生物合成和细胞蛋白质修饰以及光刺激过程的响应。在这些蛋白质中，精氨琥珀酸合酶的上聚与游离腐胺含量的增加有关，因此，在W _m B _d下具有较高的芽伸长率。R LED。钙网蛋白，热休克蛋白，质体脂质相关蛋白，泛素结合酶和紫外线B受体UVR8亚型X1的向下积累可能与LED处理下较长的芽长有关。这项研究提供了重要的数据，有关光质量对体外形态发生的影响，这是通过调节特定蛋白和游离腐胺的生物合成来实现的。C。fissilis是巴西大西洋森林中一种具有经济和生态意义的濒危木材。可以通过ProteomeXchange在标识符PXD018020下获得菲氏梭菌的非冗余蛋白数据库。