In a previous study, the sequential optimization and regulation of environmental parameters using the PhotoBiobox was demonstrated with high throughput screening tests. In this study, we estimated changes in the biovolume-based composition of a polyculture built in vitro and composed of three algal strains: Chlorella sp., Scenedesmus sp., and Parachlorella sp. This study was performed using the PhotoBiobox under different temperatures (10-36°C) and light intensities (50-700 μmol m^-2 s^-1) in air and in 5% CO₂. In 5% CO₂, Chlorella sp. exhibited better adaptation to high temperatures than in air conditions. Pearson's correlation analysis showed that the composition of Parachlorella sp. was highly related to temperature whereas Chlorella sp. and Scenedesmus sp. showed negative correlations in both air and 5% CO₂. Furthermore, light intensity slightly affected the composition of Scenedesmus sp., whereas no significant effect was observed in other species. Based on these results, it is speculated that temperature is an important factor in influencing changes in algal polyculture community structure (PCS). These results further confirm that the PhotoBiobox is a convenient and available tool of performing lab-scale experiments on PCS changes. The application of the PhotoBiobox to the study of PCS will provide new insight into polyculture-based ecology.

中文翻译：

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使用 PhotoBiobox 对改变的藻类群落结构进行高通量体外筛选。

在之前的一项研究中，通过高通量筛选测试证明了使用 PhotoBiobox 对环境参数进行顺序优化和调节。在这项研究中，我们估计了体外构建的混养生物体积组成的变化，该混养由三种藻类组成：小球藻、栅藻和副小球藻。这项研究是在不同温度 (10-36°C) 和光照强度 (50-700 μmol m ^-2 s ^-1 ) 下在空气和 5% CO ₂中使用 PhotoBiobox 进行的。在 5% CO ₂中，小球藻sp。比在空气条件下表现出更好的高温适应性。Pearson 的相关分析表明，Parachlorella sp. 的组成。与温度高度相关，而小球藻sp。和栅藻 sp。_{在空气和 5% CO 2}中均呈负相关。此外，光照强度略微影响栅藻的组成sp.，而在其他物种中没有观察到显着影响。基于这些结果，推测温度是影响藻类混养群落结构（PCS）变化的重要因素。这些结果进一步证实，PhotoBiobox 是一种方便且可用的工具，可以对 PCS 变化进行实验室规模的实验。PhotoBiobox 在 PCS 研究中的应用将为基于混养的生态学提供新的见解。