TomloxB is the main isoform of lipoxygenase associated with ripening and senescence of fruits. On the other hand, ethylene, a gaseous hormone, is essential for the regulation of ripening in climacteric fruits like tomatoes. However, the relationship between TomloxB and ethylene production has not been thoroughly studied. Therefore, we aim to assess the effect of exogenous ethylene in transgenic tomatoes that contain a silenced TomloxB gene, and subsequently evaluate lipoxygenase activity, 1-aminocyclopropane-1-carboxylic acid oxidase and ethylene production; as well as to quantify the expression of the genes encoding 1-aminocyclopropane-1-carboxylic acid oxidase and TomloxB.

中文翻译：

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转基因番茄（Solanum lycopersicum）成熟过程中的脂氧合酶及其与乙烯的关系。

TomloxB 是与果实成熟和衰老相关的脂氧合酶的主要异构体。另一方面，乙烯是一种气态激素，对于调节西红柿等更年期水果的成熟至关重要。然而，TomloxB 与乙烯生产之间的关系尚未得到深入研究。因此，我们旨在评估外源乙烯对含有沉默的TomloxB基因的转基因番茄的影响，并随后评估脂氧合酶活性、1-氨基环丙烷-1-羧酸氧化酶和乙烯生产；以及量化编码1-氨基环丙烷-1-羧酸氧化酶和TomloxB的基因的表达。