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Single-cell analysis by use of ICP-MS
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2020-08-24 , DOI: 10.1039/d0ja00194e
Sarah Theiner 1, 2, 3, 4 , Konrad Loehr 5, 6, 7, 8, 9 , Gunda Koellensperger 1, 2, 3, 4 , Larissa Mueller 7, 10, 11 , Norbert Jakubowski 7, 12
Affiliation  

This tutorial review article is highlighting the fundamentals, instrumentation, and most recent trends of single-cell analysis by use of inductively coupled plasma-mass spectrometry (ICP-MS). It is shown that metals and hetero-elements being intrinsically present in cells, taken up by cells (for instance engineered metallic nanoparticles) or binding to a cell can be detected qualitatively by existing ICP-MS technologies on a single cell level. Adding a quantitative dimension to single-cell analysis by (laser ablation-) ICP-MS requires dedicated calibration and validation strategies, which are currently being established and are being critically discussed. In a tutorial part, the ICP-MS instruments, the measurement conditions, and the sample introduction and preparation techniques are introduced. The application section focuses on the state-of-the-art of single-cell analysis in suspension, using laser ablation or (imaging) mass cytometry. Finally, future trends are critically assessed.

中文翻译:

使用ICP-MS进行单细胞分析

本教程的评论文章重点介绍了通过使用电感耦合等离子体质谱(ICP-MS)进行单细胞分析的基础知识,仪器和最新趋势。结果表明,可以通过现有的ICP-MS技术在单个细胞水平上定性检测细胞中固有存在的金属和异质元素,被细胞(例如工程化的金属纳米颗粒)吸收或与细胞结合的金属和异质元素。通过(激光烧蚀)ICP-MS在单细胞分析中增加定量范围需要专用的校准和验证策略,这些策略目前正在建立中,并且正在被严格讨论。在教程部分中,介绍了ICP-MS仪器,测量条件以及样品引入和制备技术。应用部分重点介绍使用激光消融或(成像)质谱细胞仪对悬浮液中单细胞进行分析的最新技术。最后,对未来趋势进行严格评估。
更新日期:2020-09-02
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