Single-cell analysis by use of ICP-MS,Journal of Analytical Atomic Spectrometry

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Single-cell analysis by use of ICP-MS
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2020-08-24 , DOI: 10.1039/d0ja00194e
Sarah Theiner _{1,

2,

3,

4} , Konrad Loehr _{5,

6,

7,

8,

9} , Gunda Koellensperger _{1,

2,

3,

4} , Larissa Mueller _{7,

10,

11} , Norbert Jakubowski _{7,

12}

Affiliation

This tutorial review article is highlighting the fundamentals, instrumentation, and most recent trends of single-cell analysis by use of inductively coupled plasma-mass spectrometry (ICP-MS). It is shown that metals and hetero-elements being intrinsically present in cells, taken up by cells (for instance engineered metallic nanoparticles) or binding to a cell can be detected qualitatively by existing ICP-MS technologies on a single cell level. Adding a quantitative dimension to single-cell analysis by (laser ablation-) ICP-MS requires dedicated calibration and validation strategies, which are currently being established and are being critically discussed. In a tutorial part, the ICP-MS instruments, the measurement conditions, and the sample introduction and preparation techniques are introduced. The application section focuses on the state-of-the-art of single-cell analysis in suspension, using laser ablation or (imaging) mass cytometry. Finally, future trends are critically assessed.

中文翻译：

使用ICP-MS进行单细胞分析

本教程的评论文章重点介绍了通过使用电感耦合等离子体质谱（ICP-MS）进行单细胞分析的基础知识，仪器和最新趋势。结果表明，可以通过现有的ICP-MS技术在单个细胞水平上定性检测细胞中固有存在的金属和异质元素，被细胞（例如工程化的金属纳米颗粒）吸收或与细胞结合的金属和异质元素。通过（激光烧蚀）ICP-MS在单细胞分析中增加定量范围需要专用的校准和验证策略，这些策略目前正在建立中，并且正在被严格讨论。在教程部分中，介绍了ICP-MS仪器，测量条件以及样品引入和制备技术。应用部分重点介绍使用激光消融或（成像）质谱细胞仪对悬浮液中单细胞进行分析的最新技术。最后，对未来趋势进行严格评估。

更新日期：2020-09-02

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