Melanoma a common skin tumor induced by excessive hyperplasia of abnormal melanocyte. Circular RNAs (circRNAs) play critical roles in various diseases and presented as the prognostic markers of melanoma. The present study was designed to confirm the effect of circ 0001591 on human melanoma cell growth and to elucidate its mechanism. Patient with melanoma was obtained from Shaanxi Provincial People’s Hospital. Cell viability of A2058 cell was detected by MTT assay. The expression of circ 0001591 in serum of patients with melanoma was increased. Up-regulation of circ 0001591 promoted cell growth and cell invasion, and reduced apoptotic rate of melanoma. Down-regulation of circ 0001591 reduced cell growth and cell invasion, and promoted apoptotic rate of melanoma.

Up-regulation of circ 0001591 induced PI3 K and p-Akt protein expressions in melanoma through induction of ROCK1 by suppression of miR-431-5p. Over-expression of circ 0001591 suppressed PI3 K and p-Akt protein expressions via suppression of ROCK1 in melanoma by induction of miR-431-5p. MiR-431-5p reduced the effects of circ 0001591 down-regulation on cell growth of melanoma through PI3K/AKT signal pathway. ROCK1 reduced the effects of circ 0001591 on cell growth of melanoma through PI3K/AKT signal pathway. Our findings demonstrated that circ 0001591 inhibits the progression of human melanoma through ROCK1/PI3K/AKT signal pathway by targeting ROCK1 by miR-431-5p.

黑色素瘤是一种由异常黑色素细胞过度增生引起的常见皮肤肿瘤。环状RNA（circRNA）在各种疾病中发挥关键作用，并作为黑色素瘤的预后标志物出现。本研究旨在确认 circ 0001591 对人类黑色素瘤细胞生长的影响并阐明其机制。黑色素瘤患者来自陕西省人民医院。MTT法检测A2058细胞活力。黑色素瘤患者血清中circ 0001591的表达增加。circ 0001591 的上调促进细胞生长和细胞侵袭，并降低黑色素瘤的凋亡率。circ 0001591 的下调减少了细胞生长和细胞侵袭，并促进了黑色素瘤的凋亡率。

circ 0001591 的上调通过抑制 miR-431-5p 诱导 ROCK1 来诱导黑色素瘤中 PI3 K 和 p-Akt 蛋白的表达。circ 0001591 的过表达通过诱导 miR-431-5p 抑制黑色素瘤中的 ROCK1 来抑制 PI3 K 和 p-Akt 蛋白的表达。MiR-431-5p 通过 PI3K/AKT 信号通路降低了 circ 0001591 下调对黑色素瘤细胞生长的影响。ROCK1 通过 PI3K/AKT 信号通路降低了 circ 0001591 对黑色素瘤细胞生长的影响。我们的研究结果表明，circ 0001591 通过 ROCK1/PI3K/AKT 信号通路通过 miR-431-5p 靶向 ROCK1 抑​​制人类黑色素瘤的进展。