Abstract

Here, a newly modified chitosan tetrazole was synthesized and fully characterized for CRISPR technology since by increasing the ratio of nitrogen to other elements, the interaction between the genetic material and this nanomaterial was significantly enhanced. The nanomaterial was added to HEK-293 cells for pCRISPR delivery. The nanomaterials were optimized and showed an acceptable binding ability with DNA due to its increasing zeta potential in the synthesized chitosan tetrazole in comparison with chitosan, as well as considerable transfection efficiency, more than 25%, and, thus, can be considered as a promising and also new gold-standard for the delivery of pCRISPR.

摘要

在这里，一种新修饰的壳聚糖四唑被合成并完全表征为 CRISPR 技术，因为通过增加氮与其他元素的比例，遗传材料和这种纳米材料之间的相互作用显着增强。将纳米材料添加到 HEK-293 细胞中以进行 pCRISPR 递送。由于与壳聚糖相比，合成的壳聚糖四唑中的 zeta 电位增加，纳米材料经过优化并显示出可接受的 DNA 结合能力，以及可观的转染效率，超过 25%，因此，可以被认为是一种有前途的以及提供 pCRISPR 的新金标准。