ABSTRACT

In a randomized double-blind crossover study, a canned beverage was prepared using an emulsion dispersion formulation (EM) of β-carotene and a crystal dispersion formulation (CR) of β-carotene; the beverages were ingested by human subjects daily for 2 weeks to compare the β-carotene bioavailability. EM-β-carotene enhanced the β-carotene concentrations in human plasma approximately 4-fold, but CR-β-carotene showed no statistically significant enhancement. Bioaccessibility is the ratio of the solubilized fraction to the whole amount ingested. Bioaccessibility of β-carotene from EM-β-carotene was higher than that from CR-β-carotene in an in vitro digestion test. Contrarily, β-carotene from CR-β-carotene, consists of all-trans-β-carotene, was higher than that from EM-β-carotene, consists of a mixture of cis and all-trans-β-carotene, on the uptake by intestinal Caco-2 cells, suggesting that bioaccessibility was a critical factor in β-carotene bioavailability in this study. EM-β-carotene thus has potential as a food coloring agent with value added because it enhances β-carotene bioavailability.

摘要

在一项随机双盲交叉研究中，使用β-胡萝卜素的乳液分散体配方（EM）和β-胡萝卜素的晶体分散体配方（CR）制备罐装饮料。每天将人类受试者摄入这些饮料2周，以比较β-胡萝卜素的生物利用度。EM-β-胡萝卜素将人血浆中的β-胡萝卜素浓度提高了约4倍，但CR-β-胡萝卜素未显示出统计学上的显着提高。生物可及性是溶解部分与摄入总量的比例。在体外消化测试中，EM-β-胡萝卜素中的β-胡萝卜素的生物可及性高于CR-β-胡萝卜素中的。相反，CR-β-胡萝卜素中的β-胡萝卜素由全反式组成-β-胡萝卜素比EM-β-胡萝卜素的含量高，它由顺式和全反式-β-胡萝卜素的混合物组成，对肠道Caco-2细胞的吸收有影响，表明生物可及性是β的关键因素-胡萝卜素的生物利用度。因此，EM-β-胡萝卜素具有增加食品价值的潜力，因为它可以增强β-胡萝卜素的生物利用度。