Two terpene synthases in resistant Pinus massoniana contribute to defense against Bursaphelenchus xylophilus.,Plant, Cell & Environment

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Two terpene synthases in resistant Pinus massoniana contribute to defense against Bursaphelenchus xylophilus.
Plant, Cell & Environment ( IF 6.0 ) Pub Date : 2020-08-24 , DOI: 10.1111/pce.13873
Bin Liu _{1,

2} , Qinghua Liu _{1,

2} , Zhichun Zhou _{1,

2} , Hengfu Yin ₁ , Yini Xie _{1,

2} , Yongcheng Wei ₃

Affiliation

Pine wood nematode (PWN; Bursaphelenchus xylophilus), a destructive pest of Pinus massoniana, is causing a severe epidemic of pine wilt disease in China. When invaded by PWN, resistant P. massoniana secretes an abundance of oleoresin terpenoids as a defensive strategy. However, regulatory mechanisms of this defence in resistant P. massoniana have yet to be elucidated. Here, we characterized two terpene synthase genes, α‐pinene synthase (PmTPS4) and longifolene synthase (PmTPS21), identified in resistant P. massoniana and investigate the contribution of these genes to the oleoresin defence strategy in resistant masson pines. Up‐regulation of these two genes in the stem supported their involvement in terpene biosynthesis as part of the defence against PWN. Recombinant protein expression revealed catalytic activity for the two PmTPSs, with PmTPS4 primarily producing α‐pinene, while PmTPS21 produced α‐pinene and longifolene simultaneously. The major enzymatic products of the two terpene synthases had inhibitory effects on PWN in vitro. We demonstrated that PmTPS4 and PmTPS21 played positive roles in terpene‐defence mechanisms against PWN infestation. The major products of these terpene synthases could directly inhibit the survival rate of PWN in vitro. We revealed that PmTPS21 was a novel bifunctional enzyme capable of simultaneous production of both monoterpene and sesquiterpene.

更新日期：2020-08-24

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