Efficient Cellular Internalization and Transport of Bowl‐Shaped Polydopamine Particles,Particle & Particle Systems Characterization

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Efficient Cellular Internalization and Transport of Bowl‐Shaped Polydopamine Particles
Particle & Particle Systems Characterization ( IF 2.7 ) Pub Date : 2020-08-23 , DOI: 10.1002/ppsc.202000166
Shahinur Acter ₁ , Mark Louis P. Vidallon ₁ , Simon Crawford ₂ , Rico F. Tabor ₁ , Boon Mian Teo ₁

Affiliation

In drug delivery applications, particle‐based systems have been used widely due to their physicochemical properties such as size, shape, and surface charge to achieve desirable properties in intracellular environments. The way in which nanoparticles enter a biological cell is an important factor in determining their efficacy as drug carriers, their biodistribution, and toxicity. Most research thus far has focused on the comparison of spherical and rod‐like particles on cellular internalization and transport. Here, the synthesis of bowl‐shaped polydopamine (PDA) mesoporous nanoparticles with an average diameter of 200 nm and well‐controlled radially oriented mesochannels are reported. By incubating bowl‐shaped PDA nanoparticles and spherical nanoparticles with HeLa cells, their internalization behaviors are investigated using a suite of characterization techniques. Extensive experimental results demonstrate that bowl‐shaped PDA nanoparticles adhere to the cell more efficiently and a faster rate of cellular uptake of bowl‐shaped nanoparticles compared to their spherical counterparts. Overall, the cellular internalization behavior of particles is shape‐dependent, and such information is crucial in designing nanoparticles for biomedical applications.

中文翻译：

碗形聚多巴胺颗粒的高效细胞内化和转运

在药物输送应用中，基于颗粒的系统由于其物理化学性质（如大小，形状和表面电荷）而得到广泛使用，从而在细胞内环境中获得理想的性质。纳米粒子进入生物细胞的方式是决定其作为药物载体的功效，其生物分布和毒性的重要因素。迄今为止，大多数研究都集中在比较球形和棒状颗粒在细胞内化和转运方面的作用。在此，报道了平均直径为200 nm的碗形聚多巴胺（PDA）介孔纳米颗粒的合成，并且径向取向良好的介孔通道得到了很好的控制。通过将碗形PDA纳米颗粒和球形纳米颗粒与HeLa细胞一起孵育，使用一套表征技术研究了它们的内部化行为。大量的实验结果表明，与球形的PDA纳米颗粒相比，碗形的PDA纳米颗粒可以更有效地粘附到细胞上，碗形纳米颗粒的细胞摄取速度更快。总体而言，颗粒的细胞内在行为取决于形状，而这些信息对于设计用于生物医学应用的纳米颗粒至关重要。

更新日期：2020-09-16

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