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Detection of Arcobacter species in different intestinal compartments of broiler chicken during slaughter and processing.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-08-24 , DOI: 10.1002/mbo3.1106 Antje Schönknecht 1 , Thomas Alter 1 , Greta Gölz 1
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-08-24 , DOI: 10.1002/mbo3.1106 Antje Schönknecht 1 , Thomas Alter 1 , Greta Gölz 1
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Arcobacter spp. are commonly present on meat products. However, the source of contamination on chicken meat is under dispute. Since different studies reported contradictory results on the occurrence of Arcobacter spp. inside the intestinal tract of chicken, our study examined four intestinal compartments at four significant production steps during broiler slaughter and processing in the slaughterhouse. Altogether, 157 intestinal tracts from 19 flocks were examined qualitatively and semiquantitatively applying a selective enrichment. Further verification was performed by mPCR and rpoB sequencing. Arcobacter spp. were only detected sporadically in intestinal contents after bleeding (2/32) and in none after scalding (0/32). After defeathering, Arcobacter spp. were detected in 62% (18/29) of the intestinal contents with 28% (8/29) of the duodenal, 21% (6/29) of the jejunal, 3% (1/29) of the cecal, and 55% (16/29) of the colonic samples tested positive with loads up to 24,000 MPN/g in the colonic content. Further 88% (7/8) of colonic tissue samples were tested positive. After evisceration, the prevalences (58/64) and loads of Arcobacter spp. display comparable levels in the intestinal contents like after defeathering. In conclusion, our data point out that Arcobacter spp. are most likely detected in the colonic intestinal compartment of the chicken after defeathering and evisceration. Therefore, not only cross‐contamination originating from the environment inside the slaughterhouse may cause carcass contamination with Arcobacter spp. on broiler chicken carcasses. The detection of Arcobacter spp. in duodenal and jejunal contents as well as in the colonic tissue indicates that there possibly exists an Arcobacter reservoir inside the chicken.
中文翻译：
在屠宰和加工过程中检测肉鸡不同肠腔中的杆菌。
弧菌属。通常存在于肉制品上。但是，鸡肉污染的来源尚有争议。由于不同的研究报道了关于杆菌属的发生的相互矛盾的结果。在鸡的肠道内，我们的研究在肉鸡屠宰和屠宰场加工过程中的四个重要生产步骤中检查了四个肠道隔室。总共，对19个鸡群的157个肠道进行了定性和半定量应用选择性富集的检查。通过mPCR和rpoB测序进行了进一步验证。弧菌属。仅在出血后（2/32）偶尔发现肠内容物，在烫伤后（0/32）没有检测到。解开后弧菌属。在62％（18/29）的肠内容物中检测到十二指肠，其中28％（8/29）的十二指肠，21％（6/29）的空肠，3％（1/29）的盲肠和55 ％（16/29）的结肠样本测试为阳性，结肠内容物负荷高达24,000 MPN / g。另外88％（7/8）的结肠组织样本被测试为阳性。去除内脏后，Arcobacter spp的患病率（58/64）和负荷。在解肠后，在肠内容物上显示出可比的水平。总而言之，我们的数据指出弓形杆菌spp。解粘和剔除后最有可能在鸡的结肠肠腔中检测到。因此，不仅来自屠宰场内部环境的交叉污染还可能导致Ar体菌污染car体。在肉鸡的鸡身上。弧菌属的检测。十二指肠和空肠以及结肠组织中的含量表明，鸡体内可能存在一个杆菌属贮库。
更新日期：2020-10-17
中文翻译：
在屠宰和加工过程中检测肉鸡不同肠腔中的杆菌。
弧菌属。通常存在于肉制品上。但是，鸡肉污染的来源尚有争议。由于不同的研究报道了关于杆菌属的发生的相互矛盾的结果。在鸡的肠道内，我们的研究在肉鸡屠宰和屠宰场加工过程中的四个重要生产步骤中检查了四个肠道隔室。总共，对19个鸡群的157个肠道进行了定性和半定量应用选择性富集的检查。通过mPCR和rpoB测序进行了进一步验证。弧菌属。仅在出血后（2/32）偶尔发现肠内容物，在烫伤后（0/32）没有检测到。解开后弧菌属。在62％（18/29）的肠内容物中检测到十二指肠，其中28％（8/29）的十二指肠，21％（6/29）的空肠，3％（1/29）的盲肠和55 ％（16/29）的结肠样本测试为阳性，结肠内容物负荷高达24,000 MPN / g。另外88％（7/8）的结肠组织样本被测试为阳性。去除内脏后，Arcobacter spp的患病率（58/64）和负荷。在解肠后，在肠内容物上显示出可比的水平。总而言之，我们的数据指出弓形杆菌spp。解粘和剔除后最有可能在鸡的结肠肠腔中检测到。因此，不仅来自屠宰场内部环境的交叉污染还可能导致Ar体菌污染car体。在肉鸡的鸡身上。弧菌属的检测。十二指肠和空肠以及结肠组织中的含量表明，鸡体内可能存在一个杆菌属贮库。
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