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Formation, stability and antimicrobial activity of laurel leaves essential oil (Laurus nobilis L.) particles in suspension obtained by SFEE
The Journal of Supercritical Fluids ( IF 3.4 ) Pub Date : 2020-08-24 , DOI: 10.1016/j.supflu.2020.105032
Páulia M.C. Lima Reis , Natália Mezzomo , Gean Pablo S. Aguiar , Dachamir Hotza , Deise Helena Baggio Ribeiro , Sandra Regina Salvador Ferreira , Haiko Hense

Laurel leaves essential oil is widely studied and valued due to its bioactive functions as antimicrobial, antifungal, antioxidant, antiviral, insecticide and food applications. Encapsulation may be an alternative for protection of their active components. Therefore, the objective of this work is to evaluate the influence of process variables of the Supercritical Fluid Extraction of Emulsions (SFEE) on encapsulation efficiency and, the innovative, on the stability of the particles in suspension (ZP), as also morphological characteristics (TEM), thermal stability (TGA) and finally, as a differential, antimicrobial activity (agar diffusion method and broth dilution test) of the SFEE particles in suspension. Spherical and massive microparticles were formed by SFEE, with encapsulation efficiency ranging from 80.0 % to 56.6 %, particle size (Dp) ranging from 200 to 1000 nm, and stability enhancement with increasing pH. Encapsulation improved the thermal stability of the particles and maintained the bacteriostatic and fungistatic activities of the laurel essential oil.



中文翻译:

SFEE制得的悬浮液中月桂叶精油(Laurus nobilis L.)颗粒的形成,稳定性和抗菌活性

月桂树叶子精油因其作为抗菌剂,抗真菌剂,抗氧化剂,抗病毒剂,杀虫剂和食品应用的生物活性而被广泛研究和重视。封装可能是保护其有源组件的替代方法。因此,这项工作的目的是评估乳状液超临界流体萃取(SFEE)的工艺变量对包封效率的影响,以及创新性对悬浮液颗粒(ZP)的稳定性以及形态特征的影响( TEM),热稳定性(TGA),最后作为悬浮液中SFEE颗粒的差异抗菌活性(琼脂扩散法和肉汤稀释试验)。SFEE形成球形和块状微粒,包封率从80.0%到56.6%,粒径(Dp)在200至1000 nm之间,并且随着pH值的增加而提高稳定性。包封改善了颗粒的热稳定性,并保持了月桂精油的抑菌和抑菌活性。

更新日期:2020-08-24
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