Anabolism of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator DSM 545 from Spent Coffee Grounds Oil,New Biotechnology

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Anabolism of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator DSM 545 from Spent Coffee Grounds Oil
New Biotechnology ( IF 4.5 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.nbt.2020.08.001
Haydn Rhys Ingram ₁ , James Benjamin Winterburn ₁

Affiliation

Oil extracted from spent coffee grounds (SCG) [yield 16.8 % (w/w)] was discovered to be a highly suitable carbon substrate for the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3 HV)] copolymers by Cupriavidus necator DSM 545 in the absence of any traditional 3 HV precursors. Cells cultivated in a 3 L bioreactor (batch) reached a total biomass concentration of 8.9 g L-1 with a P(3HB-co-3 HV) (6.8 mol% 3 HV) content of 89.6 % (w/w). In contrast, cells grown on sunflower oil reached a total biomass concentration of 9.4 gL-1 with a P(3HB-co-3 HV) (0.2 mol% 3 HV) content of 88.1 % (w/w). It is proposed that the organism could synthesize 3 HV monomers from succinyl CoA, an intermediate of the tricarboxylic acid (TCA) cycle, via the succinate-propionate metabolic pathway.

中文翻译：

来自废咖啡渣油的 Cupriavidus necator DSM 545 对聚（3-羟基丁酸酯-co-3-羟基戊酸酯）的合成代谢

从废咖啡渣 (SCG) 中提取的油 [产量 16.8 % (w/w)] 被发现是一种非常适合生物合成聚（3-羟基丁酸-co-3-羟基戊酸）[P(3HB-co -3 HV)] 共聚物由 Cupriavidus necator DSM 545 在不存在任何传统 3 HV 前体的情况下生成。在 3 L 生物反应器（批次）中培养的细胞总生物质浓度达到 8.9 g L-1，P(3HB-co-3 HV) (6.8 mol% 3 HV) 含量为 89.6% (w/w)。相比之下，在向日葵油上生长的细胞达到了 9.4 gL-1 的总生物量浓度，其中 P(3HB-co-3 HV) (0.2 mol% 3 HV) 含量为 88.1% (w/w)。提出该生物体可以通过琥珀酸-丙酸代谢途径从三羧酸 (TCA) 循环的中间体琥珀酰辅酶 A 合成 3 HV 单体。

更新日期：2021-01-01

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