Botrytis is a necrotrophic fungal genus of great economic importance worldwide. Together, the Botrytis species are able to infect over one thousand host plant species, including dicotyledons and monocotyledons. As the identification of Botrytis species in Brazil has mostly been based only on morphological characterization and comparisons of the rDNA ITS region, which is not informative in the genus, its diversity remains unknown. Thus, in this study we determined the diversity and prevalence of Botrytis spp. in Brazil by multilocus phylogeny. Phylogenetic reconstruction of the genus was performed using the nuclear genes glyceraldehyde-3-phosphate dehydrogenase (G3PDH), heat-shock protein 60 (HSP60) and RNA polymerase II second largest subunit (RPB2). From analyses of 56 Botrytis isolates obtained from different hosts and geographical regions in Brazil, we found that Botrytis cinerea is the most prevalent species with considerable intraspecific genetic diversity detected by nuclear genes. Two new hosts to B. cinerea and eight host never previously reported in Brazil were found. We also reported for the first time the occurrence of Botrytispseudocinerea associated with Accasellowiana (Myrtaceae). Due to the new phylogenetic positioning of Botrytispelargonii and Botrytiseucalypti, a taxonomic review of these species was suggested.

中文翻译：

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巴西 bBotrytis 物种的多样性、流行率和系统发育定位

Botrytis 是一种在世界范围内具有重要经济意义的坏死性真菌属。一起，Botrytis 物种能够感染超过一千种寄主植物物种，包括双子叶植物和单子叶植物。由于巴西贵腐菌属物种的鉴定大多仅基于 rDNA ITS 区域的形态特征和比较，在该属中没有提供信息，因此其多样性仍然未知。因此，在本研究中，我们确定了 Botrytis 属的多样性和流行率。在巴西通过多位点系统发育。该属的系统发育重建是使用核基因 3-磷酸甘油醛脱氢酶 (G3PDH)、热休克蛋白 60 (HSP60) 和 RNA 聚合酶 II 第二大亚基 (RPB2) 进行的。通过对从巴西不同宿主和地理区域获得的 56 株灰葡萄孢菌进行分析，我们发现灰葡萄孢菌是最普遍的物种，其核基因检测到的种内遗传多样性相当可观。发现了 B. cinerea 的两个新宿主和巴西以前从未报道过的八个宿主。我们还首次报道了与 Accasellowiana（桃金娘科）相关的 Botrytispseudocinerea 的发生。由于 Botrytispelargonii 和 Botrytiseucalypti 的新系统发育定位，建议对这些物种进行分类学审查。我们还首次报道了与 Accasellowiana（桃金娘科）相关的 Botrytispseudocinerea 的发生。由于 Botrytispelargonii 和 Botrytiseucalypti 的新系统发育定位，建议对这些物种进行分类学审查。我们还首次报道了与 Accasellowiana（桃金娘科）相关的 Botrytispseudocinerea 的发生。由于 Botrytispelargonii 和 Botrytiseucalypti 的新系统发育定位，建议对这些物种进行分类学审查。