Murrah buffaloes, best breed for milk production are native of Haryana state. They contributes significantly to the farmer’s income, livelihood and food (milk and meat) security, in the semi-tropical regions of North India. Johne’s disease though endemic in the domestic livestock of the country, but reports are not available in the buffaloes suffering from morbidity due to progressive weakness and diarrhoea. We estimated the status of JD in diarrhoeic buffaloes and cattle reporting at Veterinary Clinical Complex of Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India, using conventional, serological and PCR assays.141 buffaloes suffering from chronic diarrhoea were screened to estimate sero-prevalence of MAP and 50.0 % young and 53.52 % adult animals were positive. Of 14 cattle screened, none of the young and 66.6 % adult cows were positive. In buffaloes, 66.1 and 6.77 %, fecal samples were positive in microscopy and IS900 PCR, respectively. Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.Buffaloes positive for Mycobacterium avium subspecies paratuberculosis (MAP) infection had reduced total leukocyte count and lymphocytes.

穆拉水牛是牛奶生产的最佳品种，是哈里亚纳邦的本地人。它们在印度北部的半热带地区为农民的收入，生计和粮食（牛奶和肉类）安全做出了重要贡献。约翰病虽然在该国的家畜中很流行，但由于进行性无力和腹泻而发病的水牛尚无报道。我们使用常规，血清学和PCR分析方法评估了印度哈里亚纳邦希萨尔市拉拉·拉帕特·莱动物和动物科学大学兽医临床综合体在腹泻性水牛和牛中JD的状况，筛选了141例慢性腹泻水牛估计MAP的血清阳性率以及50.0％的幼年动物和53.52％的成年动物为阳性。在经过筛选的14头牛中，没有一头是年轻的，有66头。成年母牛中有6％为阳性。在水牛中，粪便样本在显微镜和IS检查中呈阳性，分别为66.1％和6.77％分别进行900 PCR。在印度哈里亚纳邦的腹泻性水牛和牛中，JD的血清感染率很高。对鸟分枝杆菌亚种副结核病（MAP）阳性的水牛减少了总白细胞计数和淋巴细胞。