Planctomycetes is a phylum of environmentally important bacteria, which also receive significant attention due to their fascinating cell biology. Access to axenic Planctomycete cultures is crucial to study cell biological features within this phylum in further detail. In this study, we characterise three novel strains, Mal52^T, Pan258 and CA54^T, which were isolated close to the coasts of the islands Mallorca (Spain) and Panarea (Italy), and from Monterey Bay, CA, USA. The three isolates show optimal growth at temperatures between 22 and 24 °C and at pH 7.5, divide by polar budding, lack pigmentation and form strong aggregates in liquid culture. Analysis of five phylogenetic markers suggests that the strains constitute two novel species within a novel genus in the family Planctomycetaceae. The strains Mal52^T (DSM 101177^T = VKM B-3432^T) and Pan258 were assigned to the species Symmachiella dynata gen nov., sp. nov., while strain CA54^T (DSM 104301^T = VKM B-3450^T) forms a separate species of the same genus, for which we propose the name Symmachiella macrocystis sp. nov.

浮霉菌是一类对环境很重要的细菌，因其令人着迷的细胞生物学而受到广泛关注。获得无菌浮霉菌培养物对于进一步详细研究该门内的细胞生物学特征至关重要。在这项研究中，我们描述了三种新菌株 Mal52 ^T 、 Pan258 和 CA54 ^T ，它们是在马略卡岛（西班牙）和帕纳雷亚岛（意大利）海岸附近以及美国加利福尼亚州蒙特利湾分离出来的。这三种分离株在 22 至 24 °C 温度和 pH 7.5 条件下表现出最佳生长，通过极芽分裂，缺乏色素沉着，并在液体培养中形成强聚集体。对五个系统发育标记的分析表明，这些菌株构成浮霉菌科新属中的两个新物种。菌株 Mal52 ^T (DSM 101177 ^T = VKMB-3432 ^T ) 和 Pan258 被归入Symmachiella dynata gen nov., sp. 种。 11 月，而菌株 CA54 ^T (DSM 104301 ^T = VKM B-3450 ^T ) 形成同一属的一个单独物种，我们建议将其命名为Symmachiella macrocystis sp。十一月