Flowering is one of the important developmental processes, and the timing of flowering can affect seed yield. Lignin is one of the main components of plant cell walls. Lack of lignin in crops will reduce its resistance to stress, which in turn threatens the production of crops. The WRKY transcription factor family is a large family in higher plants and is widely involved in various biological processes. In this study, we isolated the WRKY transcription factor WRKY184 from Brassica napus L. that can simultaneously regulate the development of stems and flowers. BnWRKY184 is a member of the IIc subfamily, localized in the nucleus and has no transcriptional activation capability. The tissue expression analysis showed that BnWRKY184 had the highest expression level in stems. Overexpression of BnWRKY184 in Arabidopsis thaliana led to early flowering and weaker stem phenotypes. The expression levels of the flowering-related genes FLOWERING LOCUS T , SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 , LEAFY and FRUITFULL were up-regulated in transgenic plants. The gene expression levels of secondary wall synthesis-related genes, such as NAC SECONDARY WALL THICKENING PROMOTING FACTOR 2 , CELLULOSE SYNTHASE 7 and CELLLOSE SYNTHASE 8 , were down-regulated in transgenic plants. Yeast one-hybrid and promoter-LUC showed that BnWRKY184 can directly bind to the promoter region of AtNST2 and inhibit its expression. Thus, BnWRKY184 simultaneously affected plant flowering and secondary wall development by regulating the transcriptional levels of genes involved in both processes, and BnWRKY184 can directly regulate the expression of AtFUL and AtNST2 , suggesting that BnWRKY184 play roles in certain signaling pathways during plant development.

中文翻译：

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来自欧洲油菜的 WRKY 转录因子 WRKY184 参与转基因拟南芥的开花和次生壁发育

开花是重要的发育过程之一，开花时间会影响种子产量。木质素是植物细胞壁的主要成分之一。作物中缺乏木质素会降低其对胁迫的抵抗力，进而威胁到作物的生产。WRKY 转录因子家族是高等植物中的一个大家族，广泛参与各种生物过程。在这项研究中，我们从欧洲油菜中分离出 WRKY 转录因子 WRKY184，它可以同时调节茎和花的发育。BnWRKY184 是 IIc 亚家族的成员，位于细胞核中，没有转录激活能力。组织表达分析表明BnWRKY184在茎中的表达水平最高。BnWRKY184 在拟南芥中的过度表达导致早期开花和较弱的茎表型。开花相关基因 FLOWERING LOCUS T 、SUPPRESSOR OF CONSTANS 1 、LEAFY 和 FRUITFULL 的表达水平在转基因植物中上调。次生壁合成相关基因，例如 NAC SECONDARY WALL THICKENING PROMOTING FACTOR 2 、 CELLULOSE SYNTHASE 7 和 CELLLOSE SYNTHASE 8 的基因表达水平在转基因植物中被下调。酵母单杂交体和启动子-LUC 表明 BnWRKY184 可以直接结合 AtNST2 的启动子区域并抑制其表达。因此，BnWRKY184 通过调节参与这两个过程的基因的转录水平同时影响植物开花和次生壁发育，