The human brain organoid is a miniature three-dimensional tissue culture that can simulate the structure and function of the brain in an in vitro culture environment. Although we consider that human brain organoids could be used to understand brain development and diseases, experimental models of human brain organoids are so highly variable that we apply artificial intelligence (AI) techniques to investigate the development mechanism of the human brain. Therefore, this study briefly reviewed commonly used AI applications for human brain organoid-magnetic resonance imaging, electroencephalography, and gene editing techniques, as well as related AI algorithms. Finally, we discussed the limitations, challenges, and future study direction of AI-based technology for human brain organoids.

人脑类器官是一种微型三维组织培养物，可以在体外培养环境中模拟大脑的结构和功能。尽管我们认为人脑类器官可用于了解大脑发育和疾病，但人脑类器官的实验模型变化很大，以至于我们应用人工智能 (AI) 技术来研究人脑的发育机制。因此，本研究简要回顾了人脑类器官磁共振成像、脑电图和基因编辑技术中常用的人工智能应用，以及相关的人工智能算法。最后，我们讨论了基于人工智能的人脑类器官技术的局限性、挑战和未来研究方向。