There are various biotic factors affecting the hatching success of loggerhead sea turtles (Caretta caretta) nesting on Mediterranean beaches. Eggs contaminated by fungi and bacteria are adversely affected in terms of hatching success. This study aims to identify Fusarium species that cause infection by sampling eggs and nest sands that failed to hatch during the 2015–2016 nesting season. Biospeedy fungal diversity kit was used to identify Fusarium species. Fungi were isolated from eggs and nest sands F. solani, F. oxysporum, F. falciforme and F. keratoplasticum were isolated from nest sand, and F. solani, F. oxysporum were isolated from egg contents. Fusarium species were determined for the first time using molecular methods for Turkey. Fusarium solani (54.55%) was observed to be dominant and F. oxysporum (40.91%) subdominant species. In addition to these dominant species, F. falciforme (1.82%) and F. keratoplasticum (2.73%) have been the other isolated species. The hatching success of the nests contaminated with Fusarium spp. was found to be 10.6% lower than in non-infected ones. Although there was no significant difference between the percentage of hatchling success in infected and non-infected nests in the 2015 season (n = 20) where the number of nests used in sampling was low, a significant difference was found between the success rates in the 2016 season (n = 41) where the number of nests used in sampling was higher (p = 0.676, p = 0.028, respectively). Nest temperature, depth and distance from the sea were higher infection rate, whereas relative humidity was lower in infected nests than in non-infected nests (p < 0.05 for all). Identifying the Fusarium species, which pose serious risks for sea turtle nests worldwide, and investigating their effects on hatching success became more of an issue. In this study, the determination of Fusarium species contaminating the eggs in Iztuzu Beach (Muğla, Turkey) and their sequence analysis was conducted for the first time.

有多种生物因素会影响在地中海海滩上筑巢的海龟（Caretta caretta）的孵化成功。在孵化成功方面，受到真菌和细菌污染的鸡蛋受到不利影响。本研究旨在通过取样在2015-2016年筑巢季节未孵化的卵和巢沙来鉴定引起感染的镰刀菌物种。Biospeedy真菌多样性试剂盒用于鉴定镰刀菌物种。真菌是从鸡蛋和巢砂分离茄病镰刀菌，尖孢镰孢，F. falciforme和F. keratoplasticum从巢砂分离，并且茄病镰刀菌，尖孢镰孢从鸡蛋中分离出来。土耳其首次使用分子方法确定了镰刀菌物种。茄枯萎病菌（54.55％）占主导地位，F占主导地位。羟孢菌（40.91％）为主要种。除了这些优势种，F。falciforme（1.82％）和˚F。圆锥角膜（2.73％）是其他分离的物种。镰刀菌污染的巢穴的孵化成功spp。被发现比未感染者低10.6％。尽管 在采样季节使用的巢数较少的2015年季节（n = 20）中，受感染和未感染巢中的孵化成功率之间没有显着差异，但在孵化中成功率之间存在显着差异2016年季节（n  = 41），其中抽样使用的巢数更高（分别为p  = 0.676，p  = 0.028）。巢的温度，深度和离海的距离较高，感染率较高，而受感染的巢中的相对湿度要低于未感染的巢（ 所有p <0.05）。鉴定镰刀菌对全世界海龟巢构成严重风险的物种，调查它们对孵化成功的影响变得更加重要。在这项研究中，首次进行了Iztuzu海滩（土耳其穆拉）污染鸡蛋的镰刀菌属物种的测定及其序列分析。