Hybrid superparamagnetic microspheres with bone-like composition, previously developed by a bio-inspired assembling/mineralization process, are evaluated for their ability to uptake and deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in therapeutically-relevant doses along with prolonged release profiles. The comparison with hybrid non-magnetic and with non-mineralized microspheres highlights the role of nanocrystalline, nanosize mineral phases when they exhibit surface charged groups enabling the chemical linking with the growth factor and thus moderating the release kinetics. All the microspheres show excellent osteogenic ability with human mesenchymal stem cells whereas the hybrid mineralized ones show a slow and sustained release of rhBMP-2 along 14 days of soaking into cell culture medium with substantially bioactive effect, as reported by assay with C2C12 BRE-Luc cell line. It is also shown that the release extent can be modulated by the application of pulsed electromagnetic field, thus showing the potential of remote controlling the bioactivity of the new micro-devices which is promising for future application of hybrid biomimetic microspheres in precisely designed and personalized therapies.

以前通过生物启发的组装/矿化过程开发的具有骨样成分的混合超顺磁性微球，其在治疗相关剂量下以及长期服用后能够吸收和递送重组人骨形态发生蛋白2（rhBMP-2）的能力得到了评估。发布配置文件。与杂化非磁性微球和非矿化微球的比较突出了纳米晶，纳米尺寸矿物相在显示表面带电基团时的作用，从而使化学键与生长因子相连，从而缓和了释放动力学。所有微球均具有对人间充质干细胞的优异成骨能力，而杂化矿物质的微球在浸入细胞培养基中14天后缓慢且持续释放rhBMP-2，具有实质的生物活性，如通过使用C2C12 BRE-Luc细胞系的测定所报道的。还显示出释放程度可以通过脉冲电磁场​​的应用来调节，从而显示出远程控制新的微型设备的生物活性的潜力，这有望在未来的精确设计和个性化疗法中应用混合仿生微球。 。