The bean weevil Acanthoscelides obtectus is one of the major storage pests of dry bean. Tepary bean (Phaseolus acutifolius) is a great source of resistance against A. obtectus to improve dry bean production. We developed two F₂ populations of Phaseolus spp. by crossing bean genotypes resistant to A. obtectus × susceptible, to (1) search for metabolites related to bean weevil resistance using an untargeted metabolomics approach and (2) understand the genetics of resistance to A. obtectus attack using the number of adults emerged from seeds as resistance criteria. The segregation of the number of adults emerged from seeds showed that the genetics of resistance (0 emerged adults = resistant, 1 or more emerged adults = susceptible) was controlled by a single dominant gene in two (resistant × susceptible) F₂ populations. From nine metabolites detected, pipecolic acid concentration was significantly higher in the testa of resistant individuals than in the testa of susceptible individuals. From a breeding point of view, (P. Saltillo × T. amarillo) F₂ is of special interest because it is derived from a cultivated tepary × cultivated common bean cross and showed 3:1 resistant vs susceptible segregation. Therefore, this F₂ is a suitable base material for breeding programmes to obtain new bean lines with good agronomic performance, seed quality and resistance to insect attack. Pipecolic acid plays a role in resistance against A. obtectus.

中文翻译：

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菜豆属两个F 2种群抗性的遗传和代谢组学。刺五加棘

象鼻虫的豆类Acanthoscelides obtectus是干豆腐的主要仓储害虫之一。三元豆（Phaseolus acutifolius）是抵抗A.obtectus的重要来源，可提高干豆的产量。我们开发了两个菜豆属的F ₂种群。通过交叉 对易感农杆菌×易感的豆类基因型进行分析，以（1）使用非靶向代谢组学方法寻找与豆象鼻虫抗性相关的代谢物，以及（2）了解对农杆菌的抗性遗传以种子出现的成虫数量作为抗药性标准。从种子中分离出的成虫数量分离表明，抗药性的遗传（0个成虫=抗药性，1个或更多的成虫=易感）受两个（抗药性×易感）F ₂种群的单一显性基因控制。从检测到的九种代谢产物中，抗药性个体的睾丸中的胡椒酸浓度显着高于易感个体的睾丸中。从一个繁殖点，（P.萨尔蒂约 ×  T.阿马里洛）的F ₂是特别感兴趣的，因为它是从一个栽培tepary衍生×栽培菜豆交叉并呈3:1耐VS易分离。因此，该F ₂是用于育种计划的合适基础材料，以获得具有良好农艺性能，种子品质和抗虫侵害性的新豆系。胡椒酸在抵抗A. obtectus中起作用。