Quantitative polymerase chain reaction (qPCR) has been extensively used in the field of mesenchymal stem cell (MSC) research to elucidate their characteristics and clinical potential by normalization of target genes against reference genes (RGs), which are believed to be stably expressed irrespective of various experimental conditions. However, the expression of RGs is also variable depending on the experimental conditions, which may lead to false or contradictory conclusions upon normalization. Due to the current lack of information for a clear list of stable RGs in bovine MSCs, we conducted this study to identify suitable RGs in bovine MSCs.

中文翻译：

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TATA 盒结合蛋白和核糖体蛋白 4 是牛间充质干细胞定量聚合酶链反应研究中标准化的合适参考基因。


定量聚合酶链反应 (qPCR) 已广泛应用于间充质干细胞 (MSC) 研究领域，通过对照参考基因 (RG) 标准化靶基因来阐明其特征和临床潜力，据信参考基因 (RG) 能够稳定表达，无论各种实验条件。然而，RGs 的表达也会根据实验条件而变化，这可能会导致标准化后错误或矛盾的结论。由于目前缺乏牛 MSC 中稳定 RG 的明确列表的信息，我们进行了这项研究来鉴定牛 MSC 中合适的 RG。