The abnormal expression of HPV16 E6/E7 activates oncogenes and/or inactivates tumor suppressor genes, resulting in the selective growth and malignant transformation of cancer cells. miR-4454 was selected by sequencing due to its abnormal high expression in HPV16 E6/E7 positive CaSki cell compared with HPV16 E6/E7 negative C33A cell. Overexpression of miR-4454 enhances cervical cancer cell invasion and migration. ABHD2 and NUDT21 is identified as a target gene of miR-4454.The effects of ABHD2 and NUDT21 on migration and invasion of CaSki and C33A cells were determined. The dual luciferase and RT-qPCR assays confirmed that miR-4454 might regulate its targets ABHD2 and NUDT21 to promote the proliferation, invasion and migration, whereas, inhibit the apoptosis in CaSki and C33A cells.

中文翻译：

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HPV16 E6/E7 上调的 miR-4454 通过靶向宫颈癌中的 ABHD2/NUDT21 促进侵袭和迁移。

HPV16 E6/E7的异常表达激活癌基因和/或灭活抑癌基因，导致癌细胞选择性生长和恶性转化。与HPV16 E6/E7阴性C33A细胞相比，miR-4454在HPV16 E6/E7阳性CaSki细胞中异常高表达，通过测序筛选出miR-4454。miR-4454的过表达增强宫颈癌细胞侵袭和迁移。ABHD2和NUDT21被鉴定为miR-4454的靶基因。确定ABHD2和NUDT21对CaSki和C33A细胞迁移和侵袭的影响。双荧光素酶和 RT-qPCR 检测证实 miR-4454 可能调节其靶标 ABHD2 和 NUDT21 以促进增殖、侵袭和迁移，同时抑制 CaSki 和 C33A 细胞的凋亡。