The present experiment was conducted to determine whether water extracts of lemon balm and oregano could suppress anthracnose in strawberry and to identify the important secondary metabolites responsible for such activity. Runner plants of three strawberry cultivars (Fragaria × ananassa Duch. ‘Sachinoka’, ‘Akihime’, and ‘Tochiotome’) were treated with water extracts (20%, w/v) of lemon balm (Melissa officinalis L.) and oregano (Origanum vulgare L.) separately and inoculated with Colletotrichum gloeosporioides (C. fructicola, CG1). Two weeks after CG1 inoculation, it was observed that the shoots and roots of the herb extract-treated plants had lower disease incidences and indices compared to those of the control regardless of the cultivar. Consequently, dry weights of the shoots of all the cultivars treated with the herb extracts were observed to be heavier than in the control; similarly, heavier dry weights of roots were also observed in herb extract-treated plants in ‘Sachinoka’ and ‘Tochiotome’. Upon analyzing the results of ultra-performance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS), rosmarinic acid and luteolin in lemon balm and apigenin and protocatechuic acid in oregano were identified to be the metabolites with the highest concentration in their respective plants. In addition, the antifungal effect of all these compounds against CG1 was confirmed by in vitro tests. Thus, it can be concluded that water extracts of lemon balm and oregano could suppress anthracnose in strawberry plants, and the four identified compounds in the extracts could play key roles in the antifungal properties of these herbs.

进行本实验以确定柠檬香脂和牛至的水提取物是否能抑制草莓中的炭疽病，并确定引起这种活性的重要次生代谢产物。三个草莓品种转轮植物（草莓× ananassa杜赫。“Sachinoka”，“章姬”和“Tochiotome”）与水提取物处理（20％，W / V）香蜂草（香蜂草L.）和牛至（牛至L.）单独和与接种的炭疽病菌（C.腐病菌，CG1）。接种CG1后两周，观察到与草药相比，用草药提取物处理过的植物的芽和根与对照组相比具有较低的发病率和指数。因此，观察到用草药提取物处理过的所有品种的芽的干重都比对照重。同样，在“ Sachinoka”和“ Tochiotome”中用草药提取物处理过的植物中，根的干重也更大。通过分析超高效液相色谱-串联质谱仪（UPLC-MS / MS）的结果，发现柠檬香脂中的迷迭香酸和木犀草素以及牛至中的芹菜素和原儿茶酸是各自植物中浓度最高的代谢物。此外，体外测试。因此，可以得出结论，柠檬香脂和牛至的水提取物可以抑制草莓植物中的炭疽病，并且提取物中的四种已鉴定化合物可以在这些草药的抗真菌特性中发挥关键作用。