Intumescence injury of tomato leaves is a physiological disorder in which white surface spots rise on the abaxial side of the leaf and then turn brown; when it worsens, the leaf dies. Scanning electron microscope observations showed individual cells were enlarged and raised due to intumescence injury. Vein epidermal cells and stomatal guard cells were also enlarged. Hypertrophy was observed by light microscopy in epidermal cells, spongy tissue cells, and palisade tissue cells. In thin leaf sections prepared with Technovit 7100 resin, the resin peeled from the epidermal surface on the adaxial and abaxial sides of normal leaf areas. However, resin did not peel off from the enlarged epidermis in areas of intumescence. Transmission electron microscopy imaging of the cuticular layer in the epidermal cells affected by intumescence injury showed disappearance of the outermost wax layer, abnormalities in intermediate layer, and decrease in cuticular layer. Toluidine blue O staining was used to identify abnormalities in the cuticular layer and the area of intumescence, which was stained purple. The percentage of staining area relative to total area of the leaf was used to quantify the degree of intumescence. In experiments using incubators under low-UV conditions, intumescence injury was less likely to occur in young leaves or fully expanded leaves and more likely to occur in expanding leaves. In addition, the water content of the leaves in which intumescence injury occurred was lower than that in normal leaves. Intumescence injury occurred less frequently under conditions of 70% relative humidity than under conditions of 90% relative humidity. UV and relative humidity are known to affect the development of the cuticular layer, and intumescence injury was severe under conditions in which the cuticular layer had difficulty developing. Our findings showed that intumescence injury of leaves was accompanied by abnormal cuticular layer, and suggest that occurrence of intumescence injury may be reduced under conditions in which the cuticular layer is well developed, such as with UV irradiation and low relative humidity.

番茄叶片的肿胀损伤是一种生理性疾病，其中白色的表面斑点在叶片的背面上升，然后变成褐色。当它恶化时，叶子就死了。扫描电子显微镜观察显示，由于肿胀损伤，单个细胞增大和升高。静脉表皮细胞和气孔保卫细胞也增大。通过光学显微镜在表皮细胞，海绵组织细胞和栅栏组织细胞中观察到肥大。在用Technovit 7100树脂制备的薄叶片切片中，该树脂从正常叶片区域的正反面的表皮表面剥离。但是，在膨胀区域，树脂没有从扩大的表皮上剥离。受到肿胀损伤影响的表皮细胞中表皮层的透射电镜观察表明，最外层蜡层消失，中间层异常，表皮层减少。甲苯胺蓝O染色用于鉴定表皮层和肿胀区域的异常，并染色为紫色。相对于叶的总面积的染色面积百分比用于量化膨胀程度。在低紫外线条件下使用培养箱的实验中，膨胀损伤不太可能发生在幼叶或完全膨胀的叶片中，而更可能发生在膨胀的叶片中。此外，发生膨胀损伤的叶片的水分含量低于正常叶片。在相对湿度为70％的条件下发生膨胀损伤的频率比在相对湿度为90％的条件下发生的频率更低。已知紫外线和相对湿度会影响表皮层的发育，并且在表皮层难以发育的条件下，膨胀性损伤是严重的。我们的研究结果表明，叶片的膨胀损伤伴随着异常的表皮层，并表明在表皮层发育良好的条件下（如紫外线辐射和相对湿度较低），可以减少膨胀损伤的发生。在表皮层难以发育的情况下，膨胀损伤严重。我们的研究结果表明，叶片的膨胀损伤伴随着异常的表皮层，并表明在表皮层发育良好的条件下（如紫外线辐射和相对湿度较低），可以减少膨胀损伤的发生。在表皮层难以发育的情况下，膨胀损伤严重。我们的研究结果表明，叶片的膨胀损伤伴随着异常的表皮层，并表明在表皮层发育良好的条件下（如紫外线辐射和相对湿度较低），可以减少膨胀损伤的发生。