Cymbidium is one of the largest orchid genera and it is famous for its ornamental, cultural, and economic value. Many Cymbidium species have an elegant flower fragrance, including C. faberi, C. goeringii, C. ensifolium, C. kanran, C. sinense, and so on. Although the components of the flower fragrance have been identified in many orchids, the molecular mechanism of their formation and regulation has not been explored. As one of the main components of flower fragrance, methyl jasmonate (MeJA) has been selected and the biosynthesis pathway was elucidated in some Cymbidium orchids. In order to modify the traits of flower fragrance in Cymbidium orchids, the underlying regulatory mechanisms need to be identified. In this study, four MYB transcription factors screened from RNA-seq results of C. faberi were successfully cloned and molecularly characterized. The activation of these CfMYBs with CfAOC and CfJMT promoters suggested that they may participate in the regulation of MeJA formation in C. faberi. This result could provide molecular support for the genetic modification and breeding of new C. faberi cultivars.

大花ym兰是最大的兰花属之一，以观赏，文化和经济价值而闻名。许多大花蕙兰品种有优雅的花香味，其中包括C.蕙兰，春兰，建兰，C.寒兰，墨兰， 等等。尽管已经在许多兰花中鉴定出花香的成分，但尚未探讨其形成和调控的分子机理。作为花香的主要成分之一，选择了茉莉酸甲酯（MeJA），并阐明了一些兰花兰的生物合成途径。为了改变大花ym兰兰花中花香的特性，需要确定潜在的调控机制。在这项研究中，四个MYB转录因子从RNA-SEQ结果筛选C.蕙兰被成功地克隆和分子表征。这些激活CfMYBs与CfAOC和CfJMT启动子暗示他们可能参与了C. faberi中MeJA的调控。该结果可为新的梭梭（C. faberi）品种的遗传修饰和育种提供分子支持。