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Facile synthesis of Cu-CuFe2O4 nanozymes for sensitive assay of H2O2 and GSH.
Dalton Transactions ( IF 3.5 ) Pub Date : 2020-08-22 , DOI: 10.1039/d0dt02395g Fan Xia 1 , Qiaofang Shi 1 , Zhaodong Nan 1
Dalton Transactions ( IF 3.5 ) Pub Date : 2020-08-22 , DOI: 10.1039/d0dt02395g Fan Xia 1 , Qiaofang Shi 1 , Zhaodong Nan 1
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Artificial enzymes have drawn substantial research interest from the scientific community due to their advantages over natural enzymes. However, majority of artificial enzymes exhibit low affinity towards H2O2, which means that a high H2O2 concentration is needed for the oxidation of a substrate such as 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-colored oxTMB. With this concern, Cu-CuFe2O4 was facilely synthesized, wherein, Cu0 accelerates the redox capacity of Cu-CuFe2O4 as well as the electron transfer between CuFe2O4 and H2O2. These materials induce excellent activity as a peroxidase. Cu-CuFe2O4 shows high affinity towards H2O2 with lower Michaelis–Menten constant (Km) than the reported values for ferrites and Horseradish enzyme (HRP). Moreover, it took only 5 min to detect hydrogen peroxide (H2O2) and glutathione (GSH) through a colorimetric assay using Cu-CuFe2O4. Compared with CuFe2O4, the limit of detection (LOD) is about 90-fold lower for H2O2 using Cu-CuFe2O4. In addition, Cu-CuFe2O4 shows high stability as a nanozyme. Thus, the mechanism of the peroxidase-like nanozyme Cu-CuFe2O4 is proposed.
中文翻译：
简便合成Cu-CuFe2O4纳米酶，用于H2O2和GSH的灵敏测定。
人造酶由于其优于天然酶的优点而引起了科学界的广泛研究兴趣。然而，大多数人造酶对H 2 O 2的亲和力很低，这意味着将3,3'，5,5'-四甲基联苯胺（TMB）等底物氧化为高浓度的H 2 O 2是必需的。蓝色oxTMB。考虑到这一点，容易地合成了Cu-CuFe 2 O 4，其中，Cu 0促进了Cu-CuFe 2 O 4的氧化还原能力以及CuFe 2 O 4和H 2 O之间的电子转移。2。这些物质作为过氧化物酶具有出色的活性。Cu-CuFe 2 O 4对H 2 O 2具有高亲和力，其Michaelis-Menten常数（ K m）低于铁氧体和辣根酶（HRP）的报道值。此外，通过使用Cu-CuFe 2 O 4的比色测定，仅用5分钟即可检测到过氧化氢（H 2 O 2）和谷胱甘肽（GSH）。与CuFe 2 O 4相比，使用Cu-CuFe 2 O的H 2 O 2的检出限（LOD）低约90倍4。另外，Cu-CuFe 2 O 4作为纳米酶显示出高稳定性。因此，提出了过氧化物酶样纳米酶Cu-CuFe 2 O 4的机理。
更新日期：2020-09-22
中文翻译：
简便合成Cu-CuFe2O4纳米酶，用于H2O2和GSH的灵敏测定。
人造酶由于其优于天然酶的优点而引起了科学界的广泛研究兴趣。然而，大多数人造酶对H 2 O 2的亲和力很低，这意味着将3,3'，5,5'-四甲基联苯胺（TMB）等底物氧化为高浓度的H 2 O 2是必需的。蓝色oxTMB。考虑到这一点，容易地合成了Cu-CuFe 2 O 4，其中，Cu 0促进了Cu-CuFe 2 O 4的氧化还原能力以及CuFe 2 O 4和H 2 O之间的电子转移。2。这些物质作为过氧化物酶具有出色的活性。Cu-CuFe 2 O 4对H 2 O 2具有高亲和力，其Michaelis-Menten常数（ K m）低于铁氧体和辣根酶（HRP）的报道值。此外，通过使用Cu-CuFe 2 O 4的比色测定，仅用5分钟即可检测到过氧化氢（H 2 O 2）和谷胱甘肽（GSH）。与CuFe 2 O 4相比，使用Cu-CuFe 2 O的H 2 O 2的检出限（LOD）低约90倍4。另外，Cu-CuFe 2 O 4作为纳米酶显示出高稳定性。因此，提出了过氧化物酶样纳米酶Cu-CuFe 2 O 4的机理。
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