Synergistic effects of ectomycorrhizal (ECM) fungal inoculation and biochar augmentation on plant growth, nutrient uptake and soil enzymes were investigated. A nursery experiment was conducted to assess the influence of ECM fungi (Suillus indicus and S. sibiricus) and biochar amendment (2% v/v) on the growth of Pinus wallichiana seedlings. Mycorrhizal colonisation significantly increased in biochar-amended soil compared to without biochar. Inoculation with ECM fungi in biochar-amended soils significantly increased the growth, biomass and phosphorus and nitrogen uptake of P. wallichiana seedlings compared with control as well as without biochar. The physicochemical properties of the soil were improved due to biochar augmentation and ECM inoculation. Activities of soil enzymes such as acid phosphatase, urease, dehydrogenase and protease were significantly increased in biochar-amended soil along with ECM fungal inoculation. These results suggest that combined use of ECM fungal inoculation and biochar amendment had a positive impact on growth, nutrient uptake and mycorrhizal colonisation of P. wallichiana seedlings. Also, biochar prepared from pine needles has potential for enhancing plant growth and soil fertility.

中文翻译：

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生物炭增强可改善外生菌根定植、植物生长和土壤肥力

研究了外生菌根 (ECM) 真菌接种和生物炭增加对植物生长、养分吸收和土壤酶的协同作用。进行了一项苗圃试验，以评估 ECM 真菌（Suillus indicus 和 S. sibiricus）和生物炭改良剂（2% v/v）对松树幼苗生长的影响。与没有生物炭的土壤相比，生物炭改良土壤中的菌根定植显着增加。与对照和没有生物炭的土壤相比，在生物炭改良土壤中接种 ECM 真菌显着增加了 P. wallichiana 幼苗的生长、生物量和磷和氮的吸收。由于生物炭的增加和 ECM 接种，土壤的理化性质得到了改善。土壤酶活性，如酸性磷酸酶、脲酶、随着ECM真菌接种，生物炭改良土壤中脱氢酶和蛋白酶显着增加。这些结果表明，联合使用 ECM 真菌接种和生物炭改良剂对 P. wallichiana 幼苗的生长、养分吸收和菌根定植具有积极影响。此外，由松针制备的生物炭具有促进植物生长和土壤肥力的潜力。