Abstract A Novel chemosensor derived from 4- aminoantipyrine and 9- anthracene carboxaldehyde (Aminoantipyrinoanthracenyl imine) (AAPAI) was synthesized by microwave irradiation in a greener approach and characterized by FT-IR, 1H- NMR and HR-LCMS mass spectrum. The specific selectivity in sensing of Cd2+ was effectively studied by colorimetric and fluorescence technique. The chemosensor display strong “turn-on” fluorescence and promising selectivity towards sensing of Cd2+.The sensing was confirmed by UV–Visible spectrum with an isobestic point at 430 nm. AAPAI forms 1:1 complex with Cd2+ which was further confirmed by HR-LCMS mass spectrum and Job's plot analysis. The mechanism of sensing is based on Photo induced electron transfer (PET) and Chelation enhanced fluorescence (CHEF). When Cd2+ binds with AAPAI the PET process gets inhibited thereby enhancing the fluorescence intensity and CHEF takes place due to the binding of Cd2+ with AAPAI. The reversibility of AAPAI was analyzed by EDTA and the detection limit was found out to be 0.02 μM. Real sample analysis was performed by using AAPAI and various water samples. DFT study supports the four coordination of Cd2+ with AAPAI. Fluorescence cell imaging of this Cd2+ complex acts as a supporting evidence of the fluorescent nature in L929 cells which is anchored by the results of test strips study.

中文翻译：

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用于 Cd2+ 的高选择性开启荧光化学传感器的绿色开发 - 细胞成像和试纸研究

摘要 通过微波辐射以更环保的方式合成了一种源自 4-氨基安替比林和 9- 蒽甲醛 (Aminoantipyrinoanthracenyl imine) (AAPAI) 的新型化学传感器，并通过 FT-IR、1H-NMR 和 HR-LCMS 质谱进行表征。通过比色和荧光技术有效地研究了传感 Cd2+ 的特异性选择性。化学传感器显示出强烈的“开启”荧光和对 Cd2+ 传感的有希望的选择性。该传感通过具有 430 nm 等吸收点的 UV-可见光谱证实。AAPAI 与 Cd2+ 形成 1:1 复合物，这通过 HR-LCMS 质谱和 Job 绘图分析得到进一步证实。传感机制基于光致电子转移 (PET) 和螯合增强荧光 (CHEF)。当 Cd2+ 与 AAPAI 结合时，PET 过程受到抑制，从而增强荧光强度，并且由于 Cd2+ 与 AAPAI 结合而发生 CHEF。AAPAI 的可逆性通过 EDTA 进行分析，发现检测限为 0.02 μM。使用 AAPAI 和各种水样进行实际样品分析。DFT 研究支持 Cd2+ 与 AAPAI 的四个协调。这种 Cd2+ 复合物的荧光细胞成像作为 L929 细胞荧光性质的支持证据，由试纸研究的结果确定。DFT 研究支持 Cd2+ 与 AAPAI 的四个协调。这种 Cd2+ 复合物的荧光细胞成像作为 L929 细胞荧光性质的支持证据，由试纸研究的结果确定。DFT 研究支持 Cd2+ 与 AAPAI 的四个协调。这种 Cd2+ 复合物的荧光细胞成像作为 L929 细胞荧光性质的支持证据，由试纸研究的结果确定。