Garlic (Allium sativum L.) is one of the crops whose economic importance has increased considerably in recent years in Brazil. The use of plant growth-promoting bacteria (PGPB) is a useful alternative for reducing the cost of agricultural inputs and, consequently, for increasing productivity. Therefore, the main objective of this work was to isolate and evaluate potential growth promoters in plants and plant pathogenic fungi growth inhibitors using endophytic bacteria from garlic roots and bacteria from the Agricultural Microbiology Culture Collection at the Federal University of Lavras. Besides verifying improvements in the growth and physiology of garlic meristems grown in vitro under the action of PGPB. Forty-eight endophytic bacteria were identified using matrix-assisted laser desorption/ionization–time of flight mass spectrometry based on the protein profile of each isolate. Four isolates were chosen according to their ability to fix nitrogen, to produce auxin and solubilize phosphate. The cultivation of garlic meristems in tissue culture with these bacteria was established at a population level of 10⁶ CFU/mL. The evaluated criteria were: (1) the colonization capacity of the bacteria inside the garlic plants determined through scanning electron microscopy; (2) the chlorophyll content; and (3) the growth of garlic plants in vitro post-PGPB inoculation. Volatiles emitted by those isolates inhibited fungi growth. The inoculation of garlic meristems with Enterobacter cloacae and Burkholderia cepacia promoted an improvement in the growth and physiological attributes of garlic, indicating the feasibility of their application as plant growth promoters for commercial cultivation.

大蒜（Allium sativum L.）是近年来巴西经济重要性显着提高的作物之一。使用植物生长促进细菌（PGPB）是降低农业投入成本并因此提高生产率的有用替代方法。因此，这项工作的主要目的是使用大蒜根部的内生细菌和拉夫拉斯联邦大学农业微生物学培养物中的细菌，分离和评估植物和植物病原性真菌生长抑制剂中潜在的生长促进剂。除了验证体外培养的大蒜分生组织的生长和生理学改善在PGPB的作用下。根据每种分离物的蛋白质谱，使用基质辅助激光解吸/电离飞行时间质谱仪鉴定了48种内生细菌。根据它们固定氮，生产生长素和溶解磷酸盐的能力选择了四个分离株。用这些细菌在组织培养中培养大蒜分生组织的种群水平为10 ⁶ CFU / mL。评价标准为：（1）通过扫描电子显微镜确定的大蒜植物内细菌的定殖能力；（2）叶绿素含量；（3）PGPB接种后大蒜植物的体外生长。这些分离物散发的挥发物抑制了真菌的生长。大蒜分生组织的接种阴沟肠杆菌和洋葱伯克霍尔德氏菌促进了大蒜的生长和生理特性的改善，表明将其用作商业种植植物生长促进剂的可行性。