Corynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by benzyl alcohol, could be used as an efficient tunable expression system. In initial attempts, this promoter failed to function in a recombinant expression system. This was remedied by extending the original genetic context of the promoter, generating a new version Pcat-B. The Pcat-B transcription initiation site, its critical active regions, and its effect of inducers were fully characterized resulting in tunable expression. This approach proved to be very efficient in producing a pharmaceutical protein, N-terminal pro-brain natriuretic peptide (NT-proBNP). Production of approximately 440.43 mg/L NT-proBNP was achieved with the Pcat-B expression system demonstrating its application for controllable pharmaceutical protein production in C. glutamicum.

中文翻译：

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谷氨酸棒杆菌重组蛋白过表达的苯甲醇诱导启动子的鉴定、修复和表征

谷氨酸棒杆菌是生产多种生物商品的重要工业生物。我们在谷氨酸棒杆菌中发现了一个由 NCgl2319 基因编码的启动子，该启动子可由苯甲醇诱导，可用作有效的可调表达系统。在最初的尝试中，该启动子未能在重组表达系统中发挥作用。这是通过扩展启动子的原始遗传背景来解决的，生成新版本 Pcat-B。Pcat-B 转录起始位点、其关键活性区域及其诱导物的作用得到了充分表征，从而实现了可调节的表达。事实证明，这种方法在产生药物蛋白质 N 端脑钠肽前体 (NT-proBNP) 方面非常有效。产量约为 440。