A-kinase anchoring protein 350 (AKAP350) is a centrosomal/Golgi scaffold protein, critical for the regulation of microtubule dynamics. AKAP350 recruits end-binding protein 1 (EB1) to the centrosome in mitotic cells, ensuring proper spindle orientation in epithelial cells. AKAP350 also interacts with p150^glued, the main component of the dynactin complex. In the present work, we found that AKAP350 localized p150^glued to the spindle poles, facilitating p150^glued/EB1 interaction at these structures. Our results further showed that the decrease in AKAP350 expression reduced p150^glued localization at astral microtubules and impaired the elongation of astral microtubules during anaphase. Overall, this study provides mechanistic data on how microtubule regulatory proteins gather to define microtubule dynamics in mitotic cells.

A激酶锚定蛋白350（AKAP350）是一种中心体/高尔基体支架蛋白，对微管动力学的调节至关重要。AKAP350在有丝分裂细胞中向中心体募集末端结合蛋白1（EB1），以确保上皮细胞中纺锤体的正确定向。AKAP350还相互作用与P150^胶合，所述dynactin复合物的主要成分。在当前的工作中，我们发现AKAP350将p150^胶粘在主轴上，从而促进了p150^胶粘/ EB1在这些结构上的相互作用。我们的结果进一步表明，AKAP350表达的降低可减少p150^胶合定位在星状微管，并在后期损害星状微管的伸长。总的来说，这项研究提供了有关微管调节蛋白如何聚集以定义有丝分裂细胞中微管动力学的机制数据。