The accumulation of short-chain carboxylic acids (SCCAs), such as acetic acid (C₂), propionic acid (C₃), butyric acid (C₄), and valeric acid (C₅), produced by acetogens in the anaerobic digestion (AD) process hampers the maintenance of stable AD processes in biomethane production. The conversion of various SCCAs to the corresponding alcohols can be a good solution not only for utilizing abandoned or harmful SCCAs and producing useful alcohols but also for increasing the efficiency of the biogas production process. ACT01_02765 (acyl-CoA transferase) from Megasphaera hexanoica quickly and easily converted C₂-C₈ carboxylic acids into the corresponding alcohols in Escherichia coli with AdhE2 (alcohol dehydrogenase) from Clostridium acetobutylicum. E. coli (ACT01_02765 and AdhE2) converted carboxylic acids to the corresponding alcohols with a conversion yield that was approximately 40 times higher and a conversion rate that was approximately 10–50 times faster than those of E. coli (Ptb-Buk and AdhE2). The enzymatic machinery in E. coli (ACT01_02765 and AdhE2) is effective for carboxylic acids of different carbon chain lengths, resulting in the production of propanol, butanol, pentanol, hexanol, heptanol, and octanol.

乙酸在厌氧消化中产生的短链羧酸（SCCA）的积累，例如乙酸（C ₂），丙酸（C ₃），丁酸（C ₄）和戊酸（C ₅）。 （AD）工艺妨碍了生物甲烷生产中稳定AD工艺的维持。各种SCCA转化为相应的醇不仅是利用废弃的或有害的SCCA和生产有用的醇的方法，而且对于提高沼气生产过程的效率都是很好的解决方案。ACT01_02765从（酰基辅酶A转移酶）巨型球菌hexanoica快速且容易地已转化的C ₂ -C ₈通过丙酮丁醇梭菌的AdhE2（醇脱氢酶）将羧酸转化为大肠杆菌中的相应醇。大肠杆菌（ACT01_02765和AdhE2）将羧酸转化为相应的醇，转化率比大肠杆菌（Ptb-Buk和AdhE2）高约40倍，转化率约10–50倍。大肠杆菌中的酶促机制（ACT01_02765和AdhE2）对于碳链长度不同的羧酸有效，从而产生了丙醇，丁醇，戊醇，己醇，庚醇和辛醇。