Abstract

This study evaluated the effects of inoculation of whole crop corn silage with a mixture of heterofermentative lactic acid bacteria (LAB) composed of Lactobacillus hilgardii and Lactobacillus buchneri on ensiling, aerobic stability, ruminal fermentation, total tract nutrient digestibility, and growth performance of beef cattle. Uninoculated control corn silage (CON) and silage inoculated with 3.0 × 10⁵ cfu g⁻¹ of LAB containing 1.5 × 10⁵ cfu g⁻¹ of L. hilgardii CNCM I-4785 and 1.5 × 10⁵ cfu g⁻¹ of L. buchneri NCIMB 40788 (INOC) were ensiled in silo bags. The pH did not differ (P > 0.05) between the two silages during ensiling but was greater (P < 0.001) for CON than INOC after 14 d of aerobic exposure (AE). Neutral detergent insoluble crude protein (NDICP) content (% of DM and % of CP basis) of terminal INOC silage was greater (P ≤ 0.05) than that of CON. In terminal silage, concentrations of total VFA and acetate were greater (P < 0.001), while water-soluble carbohydrates were lower (P < 0.001) for INOC than CON. Yeast and mold counts were lower for INOC than CON (P ≤ 0.001) in both terminal and aerobically exposed silages. The stability of INOC was greater (P < 0.001) than that of CON after 14 d of AE. Ruminal fermentation parameters and DMI did not differ (P > 0.05) between heifers fed the two silages, while there was a tendency (P ≤ 0.07) for lower CP and starch digestibility for heifers fed INOC than CON. Total nitrogen (N) intake and N retention were lower (P ≤ 0.04) for heifers fed INOC than CON. Dry matter intake as a percentage of BW was lower (P < 0.04) and there was a tendency for improved feed efficieny (G:F; P = 0.07) in steers fed INOC vs. CON silage. The NE_m and NE_g contents were greater for INOC than CON diets. Results indicate that inoculation with a mixture of L. hilgardii and L. buchneri improved the aerobic stability of corn silage. Improvements in G:F of growing steers fed INOC silage even though the total tract digestibility of CP and starch tended to be lower for heifers fed INOC are likely because the difference in BW and growth requirements of these animals impacted the growth performance and nutrient utilization and a greater proportion of NDICP in INOC than CON.

中文翻译：

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玉米青贮饲料接种希氏乳杆菌和布氏乳杆菌对生长肉牛青贮质量、需氧稳定性、养分消化率和生长性能的影响。

摘要

本研究评估了用由希格氏乳杆菌和布氏乳杆菌组成的异发酵乳酸菌 ( LAB )混合物接种整株玉米青贮饲料对青贮、有氧稳定性、瘤胃发酵、全道养分消化率和肉牛生长性能的影响。 . 未接种的对照玉米青贮饲料 ( CON ) 和接种 3.0 × 10 ⁵ cfu g ^-1 LAB 的青贮饲料，其中含有 1.5 × 10 ⁵ cfu g ^-1的L. hilgardii CNCM I-4785 和 1.5 × 10 ⁵ cfu g ^-1的L. hilgardii CNCM I-4785 。布氏NCIMB 40788 (INOC ) 被青贮在筒仓袋中。青贮期间两种青贮饲料的 pH 值没有差异 ( P > 0.05)，但在有氧暴露 ( AE ) 14 天后，CON的 pH 值高于 INOC （P < 0.001 ）。中性去污剂不溶性粗蛋白（NDICP）含量终端INOC青贮饲料（DM和CP基础的％的％）大于（P ≤0.05）高于CON的。在终末青贮饲料中，INOC的总 VFA 和乙酸盐的浓度较高 ( P < 0.001)，而水溶性碳水化合物的浓度低于 CON ( P < 0.001)。INOC 的酵母菌和霉菌数量低于 CON（P≤ 0.001) 在终端和有氧暴露的青贮饲料中。在AE 14 d 后，INOC 的稳定性高于 CON （P < 0.001）。饲喂两种青贮饲料的小母牛的瘤胃发酵参数和 DMI 没有差异（P > 0.05），而饲喂 INOC 的小母牛的 CP 和淀粉消化率有低于 CON的趋势（P ≤ 0.07）。饲喂 INOC 的小母牛的总氮 (N) 摄入量和 N 保留量低于 ( P ≤ 0.04)。干物质摄入量占 BW 的百分比较低 ( P < 0.04)，并且饲喂 INOC 与 CON 青贮饲料的公牛的饲料效率有提高的趋势 ( G:F ; P = 0.07)。NE _m和 NE _gINOC 的含量高于 CON 饮食。结果表明，接种L. hilgardii和L. buchneri的混合物提高了玉米青贮饲料的需氧稳定性。尽管饲喂 INOC 的小母牛的 CP 和淀粉的总消化率往往较低，但饲喂 INOC 青贮饲料的生长公牛的 G:F 有所改善，这可能是因为这些动物的体重和生长需求的差异影响了生长性能和营养利用，并且INOC 中 NDICP 的比例高于 CON。