Vectorial capacity is an equation that integrates the major aspects of vector biology to predict the number of new mosquito-borne disease infections. Developed for studying transmission of malaria, vectorial capacity is rarely applied to zoonotic vector-borne diseases and is not often adjusted to account for seasonal changes in vector ecology. We used field data from Florida, United States, to expand the understanding of how vectorial capacity of Culiseta melanura (Coquillett), the primary enzootic vector of eastern equine encephalitis virus (EEEV), changes seasonally and its effect on EEEV risk. We determined parity via dissection and identified bloodmeals by PCR for field-collected Cs. melanura females from Central Florida. We used density of the vector, proportion of avian hosts fed upon, parity state of the vector, and mean temperature of the study area to quantify vectorial capacity as a function of season. The calculated values of vectorial capacity shifted significantly with season, with highest values observed in the summer with an additional peak in December. Linear regression revealed a strong positive correlation between vectorial capacity values and Florida EEEV equine cases in 2018, as well as cases reported during the last decade. The relationship between virus infections in equids and vectorial capacity lends support to the large effect that enzootic transmission has on epizootic outbreaks of zoonotic vector-borne pathogens.

中文翻译：

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Culiseta melanura（双翅目：Culicidae）的载体容量季节性变化并与佛罗里达州中部东部马脑炎病毒的流行传播有关

媒介能力是一个方程，它整合了媒介生物学的主要方面，以预测新的蚊媒疾病感染的数量。为研究疟疾传播而开发，媒介能力很少应用于人畜共患病媒介传播疾病，并且通常不会调整以考虑媒介生态学的季节性变化。我们使用来自美国佛罗里达州的现场数据，以扩展对东部马脑炎病毒 (EEEV) 的主要地方性动物媒介 Culiseta melanura (Coquillett) 的媒介能力如何季节性变化及其对 EEEV 风险的影响的理解。我们通过解剖确定了奇偶性，并通过 PCR 鉴定了现场收集的 Cs 的血粉。来自佛罗里达州中部的 melanura 雌性。我们使用了载体的密度，以鸟类宿主为食的比例，载体的奇偶状态，和研究区域的平均温度，以量化作为季节函数的矢量容量。矢量容量的计算值随季节发生显着变化，最高值出现在夏季，并在 12 月达到峰值。线性回归揭示了矢量容量值与 2018 年佛罗里达州 EEEV 马病例以及过去十年报告的病例之间存在很强的正相关性。马科动物病毒感染与媒介能力之间的关系支持地方性传播对人畜共患病媒介传播病原体的流行爆发的巨大影响。线性回归揭示了矢量容量值与 2018 年佛罗里达州 EEEV 马病例以及过去十年报告的病例之间存在很强的正相关性。马科动物病毒感染与媒介能力之间的关系支持地方性传播对人畜共患病媒介传播病原体的流行爆发的巨大影响。线性回归揭示了矢量容量值与 2018 年佛罗里达州 EEEV 马病例以及过去十年报告的病例之间存在很强的正相关性。马科动物病毒感染与媒介能力之间的关系支持地方性传播对人畜共患病媒介传播病原体的流行爆发的巨大影响。