Peripheral nerve injury is a life-changing disability with significant socioeconomic consequences. In this rat model, we propose that partial enzyme digestion can facilitate the functional recovery of a crushed nerve. The sciatic nerves were harvested and in vitro cultured with the addition of Liberase to determine the appropriate enzyme amount in the hyaluronic acid (HA) membrane. Then, the sciatic nerve of adult male Sprague-Dawley rats was exposed, crushed, and then treated with partial enzyme digestion (either 0.001 or 0.002 unit/mm² Liberase-HA membrane). The sciatic function index (SFI) for functional recovery of the sciatic nerve was evaluated. After 2 h of in vitro digestion, fascicles and axons were separated from each other, with the cells mobilized. Greater destruction of histology structures occurred in the high enzyme (Liberase-HA membrane at 0.002 unit/mm²) group at 24 h than in the low enzyme (0.001 unit/mm²) group at 48 h. In the SFI evaluation, the improvement in 0.001 unit/mm² Liberase group was significantly better than control and 0.002 unit/mm² Liberase group. Our study demonstrated that appropriate enzyme digestion had a significantly faster and earlier recovery.

中文翻译：

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部分酶消化促进大鼠压迫神经的再生

周围神经损伤是一种改变生命的残疾，具有重大的社会经济后果。在这个大鼠模型中，我们建议部分酶消化可以促进神经压迫的功能恢复。收获坐骨神经，并通过添加Liberase进行体外培养，以测定透明质酸（HA）膜中的适当酶量。然后，将成年雄性Sprague-Dawley大鼠的坐骨神经暴露，压碎，然后进行部分酶消化（0.001或0.002单位/ mm ² Liberase-HA膜）处理。评估了坐骨神经功能恢复的坐骨神经功能指数（SFI）。体外2小时后消化后，细胞被动员，束和轴突彼此分离。高酶（Liberase-HA膜的0.002单位/ mm ²）组在24小时时的组织学结构破坏程度比低酶（0.001单位/ mm ²）的组在48小时时更大。在SFI评估中，0.001单位/ mm ^2的Liberase组的改善显着优于对照组和0.002单位/ mm ^2的Liberase组。我们的研究表明适当的酶消化具有明显更快和更早的恢复。