Long non-coding RNA (lncRNA) is characterized by biological function in diverse cancers. LncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) is well acknowledged to regulate various cancers, while its role in bladder cancer remains unclear. In the present study, we aimed at probing into the impact and detailed mechanisms of KCNQ1OT1 in bladder cancer progression. In this study, we demonstrated that KCNQ1OT1 expression in bladder cancer tissues was notably up-regulated compared with in normal adjacent tissues, and KCNQ1OT1 modulated the malignant phenotypes of bladder cancer cells. Moreover, it was validated that KCNQ1OT1 could specifically bind to miR-218-5p and reduce its expression. Overexpressed miR-218-5p would inhibit the proliferation and metastasis of bladder cancer cells while facilitating apoptosis. In terms of Mechanism, Heparan Sulfate-Glucosamine 3-Sulfotransferase 3B1 (HS3ST3B1) was validated as a target gene of miR-218-5p, and could be regulated by KCNQ1OT1 indirectly. In conclusion, KCNQ1OT1 can promote the progression of bladder cancer through regulation of miR-218-5p/HS3ST3B1, which is expected to serve as a new therapeutic target for bladder cancer.

长链非编码 RNA (lncRNA) 在多种癌症中具有生物学功能。LncRNA KCNQ1 相反链/反义转录物 1 (KCNQ1OT1) 被公认为调节各种癌症，但其在膀胱癌中的作用仍不清楚。在本研究中，我们旨在探讨 KCNQ1OT1 在膀胱癌进展中的影响和详细机制。在本研究中，我们证明与正常邻近组织相比，膀胱癌组织中 KCNQ1OT1 的表达显着上调，并且 KCNQ1OT1 调节了膀胱癌细胞的恶性表型。此外，已验证 KCNQ1OT1 可以特异性结合 miR-218-5p 并降低其表达。过表达的 miR-218-5p 会抑制膀胱癌细胞的增殖和转移，同时促进细胞凋亡。在机制方面，硫酸乙酰肝素-氨基葡萄糖 3-磺基转移酶 3B1 (HS3ST3B1) 被证实是 miR-218-5p 的靶基因，并且可以被 KCNQ1OT1 间接调控。综上所述，KCNQ1OT1可以通过调控miR-218-5p/HS3ST3B1促进膀胱癌的进展，有望成为膀胱癌的新治疗靶点。