Abstract

The present study aimed to investigate the protective role of sirtuin 1 (SIRT1) and oxygen regulated protein 150 (ORP150) in a rat COPD model by inducing changes in ER stress and apoptosis. We separated 48 Sprague Dawley (SD) rats into four groups randomly: the control group, resveratrol group, COPD group and the resveratrol intervention group. Rats were challenged with cigarette smoke and lipopolysaccharide with resveratrol (a selective activator of SIRT1). The lung functions of the rats were measured and recorded. The expression levels of SIRT1 and ORP150 in lung tissues were examined by western blot and RTq PCR. The expression levels of the ER stress apoptosis-associated protein were determined .The apoptotic level of lung tissues was analyzed. The results suggest that SIRT1 attenuated apoptosis and ER stress in the lung tissues of rats with COPD. During this process, a positive correlation was identified between SIRT1 and ORP150.

摘要

本研究旨在通过诱导内质网应激和细胞凋亡的变化来研究 Sirtuin 1 (SIRT1) 和氧调节蛋白 150 (ORP150) 在大鼠 COPD 模型中的保护作用。我们将 48 只 Sprague Dawley (SD) 大鼠随机分为四组：对照组、白藜芦醇组、COPD 组和白藜芦醇干预组。大鼠用香烟烟雾和含有白藜芦醇（SIRT1 的选择性激活剂）的脂多糖进行攻击。测量并记录大鼠的肺功能。通过蛋白质印迹和RTq PCR检测肺组织中SIRT1和ORP150的表达水平。测定ER应激细胞凋亡相关蛋白的表达水平。分析肺组织的细胞凋亡水平。结果表明 SIRT1 减弱了 COPD 大鼠肺组织的细胞凋亡和内质网应激。在此过程中，发现 SIRT1 和 ORP150 之间呈正相关。