Abstract Cross-species hybridizations have been extensively used to generate animals and plants better suited for draft and food and fiber production since Roman times, and are still important in current agricultural practices with growing uses especially in aquaculture. Diagnostic tools based on marker panels with sufficient numbers of markers for accurate identification of cross-species hybrid individuals from intercrossed and backcrossed populations are increasingly necessary for practical, accurate species-purity certification and management of commercial broodstocks. Minimal numbers of di-allelic markers with species-specific alleles required to accurately identify hybrid individuals in intercrossed and advanced backcrossed populations were estimated using power analysis, and ranged from 5 to 191 (α = .05), and from 7 to 293 (α = .01), considering backcross 1 (BC1) to BC6 populations, respectively. Numbers of markers required for accurate hybrid identification observed in simulated BC1 to BC6 populations ranged from 5 to 1,131 and 7 to 8,065, considering error rates ≤ 5% and ≤ 1%, respectively. Estimated and observed numbers of diagnostic markers required for accurate hybrid identification up to four generations of backcrossing fall within practical operational limits of most commercial platforms currently available for genotyping low-density SNP marker panels. Therefore, cost-effective assay panels could be developed to provide practical tools for accurate species-purity certification.

中文翻译：

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确定识别来自不同类型间和回交种群的二倍体跨物种杂交个体的基因渗入所需的双等位基因诊断标记的最小数量

摘要 自罗马时代以来，跨物种杂交已被广泛用于产生更适合草料和食物和纤维生产的动植物，并且在当前的农业实践中仍然很重要，尤其是在水产养殖中。基于标记组的诊断工具具有足够数量的标记，用于准确识别来自交叉和回交种群的跨物种杂交个体，对于实用、准确的物种纯度认证和商业亲鱼的管理越来越必要。使用功效分析估计了在交叉和高级回交种群中准确识别杂交个体所需的具有物种特异性等位基因的双等位基因标记的最小数量，范围从 5 到 191 (α = .05)，从 7 到 293 (α = .01), 分别考虑回交 1 (BC1) 到 BC6 种群。在模拟的 BC1 到 BC6 种群中观察到的准确杂交识别所需的标记数量范围为 5 到 1,131 和 7 到 8,065，分别考虑到错误率≤ 5% 和≤ 1%。准确杂交鉴定多达四代回交所需的估计和观察到的诊断标记数量属于目前可用于对低密度 SNP 标记面板进行基因分型的大多数商业平台的实际操作限制。因此，可以开发具有成本效益的检测面板，为准确的物种纯度认证提供实用工具。131 和 7 到 8,065，分别考虑错误率≤ 5% 和≤ 1%。准确杂交鉴定多达四代回交所需的估计和观察到的诊断标记数量属于目前可用于对低密度 SNP 标记面板进行基因分型的大多数商业平台的实际操作限制。因此，可以开发具有成本效益的检测面板，为准确的物种纯度认证提供实用工具。131 和 7 到 8,065，分别考虑错误率≤ 5% 和≤ 1%。准确杂交鉴定多达四代回交所需的估计和观察到的诊断标记数量属于目前可用于对低密度 SNP 标记面板进行基因分型的大多数商业平台的实际操作限制。因此，可以开发具有成本效益的检测面板，为准确的物种纯度认证提供实用工具。