Bordetella bronchiseptica (B. bronchiseptica) causes a respiratory disease in rabbits. To determine the proteins of B. bronchiseptica in rabbits related to the disease, differentially accumulated proteins in B. bronchiseptica‐infected cells are identified by comparative proteomic analysis. Comparative proteomic analysis detects 5814 proteins and quantifies 4854 of these. Fifty eight upregulated and 38 downregulated proteins are identified in spleen tissue after B. bronchiseptica infection of rabbits (both p < 0.05). The significantly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways are ribosome, biosynthesis of amino acids, biosynthesis of amino acids, protein export, and carbon metabolism etc. (all p < 0.01). Significantly enriched KEGG pathways include ‘ocu03010 ribosome’ (a); ‘ocu00260 glycine, serine threonine metabolism’. Analyses of control and infected spleen cells detect responses to B. bronchiseptica infection. Many differentially affected proteins are evident, and reflect different biological changes and diverse subcellular localizations between control and infected spleen cells. Infection markedly alters the expressions of proteins linked to the serine protease system, with the ‘phagosome,’ ‘biosynthesis of amino acids,’ ‘glycine, serine threonine metabolism,’ ‘intestinal immune network for IgA production’, and ‘amino sugar and nucleotide sugar metabolism’ associated with B. bronchiseptica infection. The result will inform studies of responses to B. bronchiseptica infections in rabbits.

中文翻译：

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比较蛋白质组学分析揭示了兔脾脏中支气管败血杆菌感染的复杂反应。

Bordetella bronchiseptica (B. bronchiseptica)在兔子中引起呼吸系统疾病。为了确定蛋白质支气管炎博德特氏菌在相关的疾病兔子，在差分积累蛋白质支气管炎博德特氏菌感染的细胞通过比较蛋白质组学分析鉴定。比较蛋白质组学分析检测了 5814 种蛋白质并量化了其中的 4854 种。在兔支气管败血杆菌感染后的脾组织中鉴定出 58 个上调蛋白和 38 个下调蛋白（均p < 0.05）。京都基因与基因组百科全书（KEGG）通路显着丰富的有核糖体、氨基酸生物合成、氨基酸生物合成、蛋白质输出、碳代谢等。p < 0.01)。显着富集的 KEGG 通路包括“ocu03010 核糖体”（a）；'ocu00260 甘氨酸，丝氨酸苏氨酸代谢'。对照和受感染的脾细胞的分析检测对支气管败血杆菌感染的反应。许多差异影响的蛋白质是明显的，反映了对照和受感染的脾细胞之间的不同生物学变化和不同的亚细胞定位。感染显着改变了与丝氨酸蛋白酶系统相关的蛋白质的表达，包括“吞噬体”、“氨基酸的生物合成”、“甘氨酸、丝氨酸苏氨酸代谢”、“IgA 产生的肠道免疫网络”以及“氨基糖和核苷酸”与支气管败血杆菌感染相关的糖代谢。结果将为研究提供信息B.兔支气管败血病感染。